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Evidence for contribution of an internal ribosome entry site to intercellular transport of a tobamovirus

¹ Department of Virology, Moscow State University, Vorobiovy Gory Moscow 119899, Russia
² A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Vorobiovy Gory Moscow 119899, Russia

Correspondence
J. G. Atabekov
atabekov{at}genebee.msu.su

Previously, it has been shown that tobacco mosaic virus (TMV) U1 and crucifer-infecting TMV contain a 75 nt internal ribosome entry site (IRES) upstream of movement protein (MP) gene (IRES^U1_MP,75 and IRES^CR_MP,75, respectively). A movement-deficient TMV mutant, KK6, has been constructed previously [Lehto, K., Grantham, G. L. & Dawson, W. O. (1990). Virology 174, 145–157] by insertion of the second coat protein subgenomic promoter (CP SGP-2) upstream of the MP gene, in addition to the natural CP SGP-1. Here, the authors compare the efficiency of movement function expression by KK6 and a derivative, K86, obtained by insertion of IRES^CR_MP,75 between the CP SGP-2 and MP genes resulting in restoration of IRES^CR_MP,75 function in the 5'-untranslated sequence of the I₂ subgenomic RNA of K86. The data indicate that the efficiency of K86 movement was largely restored by this insertion, which was apparently due to the translation-enhancing ability of IRES^CR_MP,75.

This article has been cited by other articles:

				Yu. L. Dorokhov, P. A. Ivanov, T. V. Komarova, M. V. Skulachev, and J. G. Atabekov An internal ribosome entry site located upstream of the crucifer-infecting tobamovirus coat protein (CP) gene can be used for CP synthesis in vivo J. Gen. Virol., September 1, 2006; 87(9): 2693 - 2697. [Abstract] [Full Text] [PDF]