HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Reggeti, F. Articles by Bienzle, D. Search for Related Content PubMed PubMed Citation Articles by Reggeti, F. Articles by Bienzle, D. Agricola Articles by Reggeti, F. Articles by Bienzle, D.

Feline immunodeficiency virus subtypes A, B and C and intersubtype recombinants in Ontario, Canada

Department of Pathobiology, University of Guelph, Guelph, ON, Canada, N1G 2W1

Correspondence
D. Bienzle
dbienzle{at}uoguelph.ca

Knowledge of the geographical distribution of feline immunodeficiency virus (FIV) subtypes is important for understanding different disease courses and for vaccine design. Intersubtype recombination may develop in areas where more than one subtype is prevalent and has the potential to create new transmittable variants with novel pathogenic properties. In this study, 40 FIV-positive DNA samples were classified by sequence analysis of the LTR–gag region. Phylogenetic analysis indicated that 32 Canadian FIV isolates clustered with previously identified subtypes A, B and C and that subtype A was most frequent in Ontario. Four strains with inconsistent clade assignment were further analysed by sequencing of the env–LTR regions. Comparisons of phylogenetic trees constructed from the two different regions of the genome and analysis of similarities to reference sequences yielded classification of three samples as A/B and one as A/C intersubtype recombinants. Although the A/B recombinant samples were obtained from unrelated cats in geographically disparate regions, a common breakpoint was consistently identified within gag. In addition, there was no evidence of co-infection with parental strains of subtypes A and B as indicated by PCR-based limiting dilution assays, although these assays allowed for the identification of two different recombinant viruses co-existing in one sample. Both sequences contained the same breakpoint. These findings suggested that a new circulating recombinant FIV may be enzootic in Ontario.

The GenBank accession numbers of the sequences reported in this article are AY220046–AY220074 and AY369376–AY369384 (LTR–gag), and AY221627–AY221629 (env–LTR).

This article has been cited by other articles:

				F. Reggeti, C. Ackerley, and D. Bienzle CD134 and CXCR4 expression corresponds to feline immunodeficiency virus infection of lymphocytes, macrophages and dendritic cells J. Gen. Virol., January 1, 2008; 89(1): 277 - 287. [Abstract] [Full Text] [PDF]

				J. J. Hayward, J. Taylor, and A. G. Rodrigo Phylogenetic Analysis of Feline Immunodeficiency Virus in Feral and Companion Domestic Cats of New Zealand J. Virol., March 15, 2007; 81(6): 2999 - 3004. [Abstract] [Full Text] [PDF]

				R. J. O. Dowling and D. Bienzle Gene-expression changes induced by Feline immunodeficiency virus infection differ in epithelial cells and lymphocytes J. Gen. Virol., August 1, 2005; 86(8): 2239 - 2248. [Abstract] [Full Text] [PDF]