| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
Correspondence
Katsunori Okazaki
ko{at}vetmed.hokudai.ac.jp
Glycoprotein B (gB) is the most conserved glycoprotein of herpesviruses and plays important roles in virus infectivity. Two intervening heptad repeat (HR) sequences were found in the C-terminal half of all herpesvirus gBs analysed. A synthetic peptide derived from the HR region (aa 477–510) of bovine herpesvirus type 1 (BoHV-1) gB was studied for its ability to inhibit virus replication. The peptide interfered with cell-to-cell spread and consistently inhibited replication of BoHV-1, with a 50 % effective concentration value (EC50) of 5 µM. Inhibition of replication was obtained not only with herpesviruses including pseudorabies virus and herpes simplex virus type 1 but also partly with Newcastle disease virus. Possible mechanisms of membrane fusion inhibition by the peptide are discussed.
This article has been cited by other articles:
|
|
 |
|
  R. Akkarawongsa, N. E. Pocaro, G. Case, A. W. Kolb, and C. R. Brandt Multiple Peptides Homologous to Herpes Simplex Virus Type 1 Glycoprotein B Inhibit Viral Infection Antimicrob. Agents Chemother., March 1, 2009; 53(3): 987 - 996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. Brandt, R. Akkarawongsa, S. Altmann, G. Jose, A. W. Kolb, A. J. Waring, and R. I. Lehrer Evaluation of a {theta}-Defensin in a Murine Model of Herpes Simplex Virus Type 1 Keratitis Invest. Ophthalmol. Vis. Sci., November 1, 2007; 48(11): 5118 - 5124. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Okazaki Proteolytic cleavage of glycoprotein B is dispensable for in vitro replication, but required for syncytium formation of pseudorabies virus J. Gen. Virol., July 1, 2007; 88(7): 1859 - 1865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Bultmann, J. Teuton, and C. R. Brandt Addition of a C-Terminal Cysteine Improves the Anti-Herpes Simplex Virus Activity of a Peptide Containing the Human Immunodeficiency Virus Type 1 TAT Protein Transduction Domain Antimicrob. Agents Chemother., May 1, 2007; 51(5): 1596 - 1607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Galdiero, M. Vitiello, M. D'Isanto, A. Falanga, C. Collins, K. Raieta, C. Pedone, H. Browne, and M. Galdiero Analysis of synthetic peptides from heptad-repeat domains of herpes simplex virus type 1 glycoproteins H and B. J. Gen. Virol., May 1, 2006; 87(Pt 5): 1085 - 1097. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Li, T. J. Minova-Foster, D. D. Norton, and M. I. Muggeridge Identification of functional domains in herpes simplex virus 2 glycoprotein B. J. Virol., April 1, 2006; 80(8): 3792 - 3800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Gianni, L. Menotti, and G. Campadelli-Fiume A Heptad Repeat in Herpes Simplex Virus 1 gH, Located Downstream of the {alpha}-Helix with Attributes of a Fusion Peptide, Is Critical for Virus Entry and Fusion J. Virol., June 1, 2005; 79(11): 7042 - 7049. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
