J Gen Virol Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 85 (2004), 2149-2154; DOI 10.1099/vir.0.79954-0

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, H.-R.
Right arrow Articles by Ahn, J.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, H.-R.
Right arrow Articles by Ahn, J.-H.
Agricola
Right arrow Articles by Lee, H.-R.
Right arrow Articles by Ahn, J.-H.
© 2004 Society for General Microbiology

Short Communication

Sumoylation of the major immediate-early IE2 protein of human cytomegalovirus Towne strain is not required for virus growth in cultured human fibroblasts

Hye-Ra Lee1,2 and Jin-Hyun Ahn1

1 Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Kyonggido 440-746, Korea
2 School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea

Correspondence
Jin-Hyun Ahn
jahn{at}med.skku.ac.kr

Sumoylation of the major immediate-early IE2 protein of human cytomegalovirus has been shown to increase transactivation activity in target reporter gene assays. This study examined the role of IE2 sumoylation in viral infection. A Towne strain-based bacterial artificial chromosome clone was generated encoding a mutated form of the IE2 protein with Lys->Arg substitutions at positions 175 and 180, the two major sumoylation sites. When human fibroblast (HF) cells were infected with the reconstituted mutant virus, (i) viral growth kinetics, (ii) the accumulation of IE1 (UL123), IE2 (UL122), p52 (UL44) and pp65 (UL83) proteins and (iii) the relocalization of the cellular small ubiquitin-like modifier (SUMO)-1, p53 and proliferating cell nuclear antigen proteins into viral DNA replication compartments were comparable with those of the wild-type and the revertant virus. The data demonstrate that sumoylation of IE2 is not essential for virus growth in cultured HF cells.




This article has been cited by other articles:


Home page
 J. Virol.Home page
A. Berndt, H. Hofmann-Winkler, N. Tavalai, G. Hahn, and T. Stamminger
Importance of Covalent and Noncovalent SUMO Interactions with the Major Human Cytomegalovirus Transactivator IE2p86 for Viral Infection
J. Virol., December 15, 2009; 83(24): 12881 - 12894.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. L. Sanders, C. J. Del Rosario, E. A. White, and D. H. Spector
Internal Deletions of IE2 86 and Loss of the Late IE2 60 and IE2 40 Proteins Encoded by Human Cytomegalovirus Affect the Levels of UL84 Protein but Not the Amount of UL84 mRNA or the Loading and Distribution of the mRNA on Polysomes
J. Virol., November 15, 2008; 82(22): 11383 - 11397.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Tomoiu, A. Gravel, R. M. Tanguay, and L. Flamand
Functional Interaction between Human Herpesvirus 6 Immediate-Early 2 Protein and Ubiquitin-Conjugating Enzyme 9 in the Absence of Sumoylation.
J. Virol., October 1, 2006; 80(20): 10218 - 10228.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2004 by the Society for General Microbiology.