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1 Centro de Biología Molecular ‘Severo Ochoa’, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain
2 Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130 Madrid, Spain
Correspondence
Esteban Domingo
edomingo{at}cbm.uam.es
Foot-and-mouth disease virus (FMDV) variants adapted to BHK-21 cells showed an expanded host-cell tropism that extended to primate and human cell lines. Virus replication in human HeLa and Jurkat cells has been documented by titration of virus infectivity, quantification of virus RNA, expression of a virus-specific non-structural antigen, and serial passage of virus in the cells. Parallel serial infections of human Jurkat cells with the same variant FMDVs indicates a strong stochastic component in the progression of infection. Chimeric viruses identified the capsid as a genomic region involved in tropism expansion. These results indicate that, contrary to theoretical predictions, replication of an RNA virus in a constant cellular environment may lead to expansion of cellular tropism, rather than to a more specialized infection of the cellular type to which the virus has been adapted.
Present address: Department of Microbiology and Immunology, University of California San Francisco, 600 16th Street, San Francisco, CA 94143-2280, USA.
Present address: UCLA AIDS Institute, Microbiology, Immunology and Molecular Genetics Department, 10833 Le Conte Avenue 11-934A Factor Building, Los Angeles, CA 90095, USA.
Present address: Institut National de la Recherche Agronomique, UMR 1225, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse Cedex 3, France.
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