HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Scobie, L. Articles by Quinn, G. Search for Related Content PubMed PubMed Citation Articles by Scobie, L. Articles by Quinn, G. Agricola Articles by Scobie, L. Articles by Quinn, G.

Characterization of germline porcine endogenous retroviruses from Large White pig

¹ Department of Veterinary Pathology, Institute of Comparitive Medicine, University of Glasgow, Switchback Road, Bearsden, Glasgow G61 1QH, UK
² Immerge BioTherapeutics Incorporation, 300 Technology Square, Cambridge, MA 02139, USA

Correspondence
Linda Scobie
l.scobie{at}vet.gla.ac.uk

Porcine endogenous retroviruses (PERV) are of concern when the microbiological safety aspects of xenotransplantation are considered. Four unique isolates of PERV B have been identified previously from a lambda library constructed from genomic DNA from a Large White pig. This study shows that none of these isolates are replication competent when transfected into permissive human or pig cells in vitro, and the removal of flanking genomic sequences does not confer a human tropic replication competent (HTRC) phenotype on these PERV proviruses. Analysis of the envelope sequences revealed that PERV B demonstrated high similarity to the envelope sequences derived from replication-competent PERV, indicating that lack of replication competence does not appear to be attributable to this region of the provirus. These data complement recent findings that HTRC PERV are recombinants between the PERV A and PERV C subgroups, and that these recombinants are not present in the germline of miniature swine. Together, these results indicate that these individual PERV B proviruses are unlikely to give rise to HTRC PERV.

This article has been cited by other articles:

				Y. Martina, K. T. Marcucci, S. Cherqui, A. Szabo, T. Drysdale, U. Srinivisan, C. A. Wilson, C. Patience, and D. R. Salomon Mice transgenic for a human porcine endogenous retrovirus receptor are susceptible to productive viral infection. J. Virol., April 1, 2006; 80(7): 3135 - 3146. [Abstract] [Full Text] [PDF]

				E. Cozzi, E. Bosio, M. Seveso, M. Vadori, and E. Ancona Xenotransplantation--current status and future perspectives. Br. Med. Bull., January 1, 2006; 75-76: 99 - 114. [Abstract] [Full Text] [PDF]