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J Gen Virol 85 (2004), 2545-2553; DOI 10.1099/vir.0.80175-0

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© 2004 Society for General Microbiology

Identification of a neutralizing epitope in the {beta}E–{beta}F loop of VP1 of equine rhinitis A virus, defined by a neutralization-resistant variant

Rachel A. Stevenson{dagger}, Jin-an Huang{ddagger}, Michael J. Studdert and Carol A. Hartley

Centre for Equine Virology, School of Veterinary Science, University of Melbourne, Parkville, VIC 3010, Australia

Correspondence
Carol A. Hartley
carolah{at}unimelb.edu.au

Equine rhinitis A virus strain 393/76 (ERAV.393/76) was passaged in the presence of post-infection ERAV.393/76 equine polyclonal antiserum (EPA). Viruses with increased resistance to neutralization by EPA were obtained after 15 passages. Compared with the parent virus, five plaque-purified, neutralization-resistant mutant viruses, in addition to the non-plaque-purified viruses that were examined, had a Glu->Lys change at position 658, which is located in the predicted {beta}E–{beta}F (EF) loop of VP1. Rabbit antiserum was prepared against the isolated EF loop of ERAV.393/76 VP1 expressed as a fusion protein with glutathione S-transferase. This antiserum bound to purified ERAV.393/76 in Western blots, but not to the neutralization-resistant mutant virus or to ERAV.PERV/62, a naturally occurring ERAV strain that has a Lys residue at position 658. These results suggest that the EF loop of VP1 is involved in a neutralization epitope of ERAV.

{dagger}Present address: Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia.

{ddagger}Present address: Pfizer, Animal Health R&D, 45 Poplar Road, Parkville, VIC 3052, Australia.






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