HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ziegler, C. Articles by Neubauer, A. Search for Related Content PubMed PubMed Citation Articles by Ziegler, C. Articles by Neubauer, A. Agricola Articles by Ziegler, C. Articles by Neubauer, A.

A glycoprotein M-deleted equid herpesvirus 4 is severely impaired in virus egress and cell-to-cell spread

Christina Ziegler^1,

¹ Institute for Medical Microbiology, Infectious and Epidemic Diseases, Ludwig-Maximilians-Universität München, Veterinärstr. 13, D-80539 Munich, Germany
² Institute for Comparative Tropical Medicine and Parasitology, Ludwig-Maximilians-Universität München, Leopoldstraße 5, D-80802 Munich, Germany
³ Boehringer Ingelheim Vetmedica GmbH, D-55216 Ingelheim, Germany

Correspondence
Antonie Neubauer
toni.neubauer{at}micro.vetmed.uni-muenchen.de

To analyse the function of the equid herpesvirus 4 (EHV-4) glycoprotein M homologue (gM), two different mutated viruses (E4gM-GFP and E4gM-w) were generated. Both gM-negative EHV-4-mutants were characterized on complementing and on non-complementing cells and compared with E4RgM, a virus where gM-expression had been repaired. It was demonstrated in virus growth kinetics that deleting gM had a more dramatic influence on EHV-4 replication than expected. Extracellular infectivity was detected 9–12 h later than in EHV-4-infected Vero cells and titres were reduced up to 2000-fold. In addition, mean maximal diameters of plaques were less than 20 % of diameters of wild-type plaques. These results are in contrast to most other alphaherpesviruses, including the closely related equid herpesvirus type 1, where deletion of gM only marginally influences the ability of viruses to replicate in cell culture. Nevertheless, analysis of infected cells by electron microscopy did not reveal a specific defect for deleting gM. It was concluded that EHV-4 gM is important for more than one step in virus replication in cell culture, influencing both efficient virus egress and cell-to-cell spread.

Present address: MSD Sharp&Dohme GmbH; D-85440 Haar, Germany.

This article has been cited by other articles:

				T. Leege, W. Fuchs, H. Granzow, M. Kopp, B. G. Klupp, and T. C. Mettenleiter Effects of Simultaneous Deletion of pUL11 and Glycoprotein M on Virion Maturation of Herpes Simplex Virus Type 1 J. Virol., January 15, 2009; 83(2): 896 - 907. [Abstract] [Full Text] [PDF]

				Y. Yamagishi, T. Sadaoka, H. Yoshii, P. Somboonthum, T. Imazawa, K. Nagaike, K. Ozono, K. Yamanishi, and Y. Mori Varicella-Zoster Virus Glycoprotein M Homolog Is Glycosylated, Is Expressed on the Viral Envelope, and Functions in Virus Cell-to-Cell Spread J. Virol., January 15, 2008; 82(2): 795 - 804. [Abstract] [Full Text] [PDF]

				M. Mach, K. Osinski, B. Kropff, U. Schloetzer-Schrehardt, M. Krzyzaniak, and W. Britt The Carboxy-Terminal Domain of Glycoprotein N of Human Cytomegalovirus Is Required for Virion Morphogenesis J. Virol., May 15, 2007; 81(10): 5212 - 5224. [Abstract] [Full Text] [PDF]

				A. D. Lipinska, D. Koppers-Lalic, M. Rychlowski, P. Admiraal, F. A. M. Rijsewijk, K. Bienkowska-Szewczyk, and E. J. H. J. Wiertz Bovine Herpesvirus 1 UL49.5 Protein Inhibits the Transporter Associated with Antigen Processing despite Complex Formation with Glycoprotein M. J. Virol., June 1, 2006; 80(12): 5822 - 5832. [Abstract] [Full Text] [PDF]