| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Short Communication |
A hypermutants by differential DNA denaturation PCR
1 Unité de Rétrovirologie Moléculaire, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris cedex 15, France
2 Unité de Recherche Prévention et Thérapie moléculaires des Maladies humaines, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris cedex 15, France
Correspondence
Jean-Pierre Vartanian
jpvart{at}pasteur.fr
Virus genomes from the same family may exhibit a wide range in their DNA GC content, whereas viral hypermutants differ substantially in GC content from their parental genomes. As AT-rich DNA melts at lower temperatures than GC-rich DNA, use of a lower denaturation temperature during PCR should allow differential amplification of AT-rich genomes or variants within a quasispecies. The latter situation has been explored explicitly in a two-step process by using a series of well-defined viral sequences differing in their AT content. Firstly, the lowest denaturation temperature (Tp) that allowed amplification of the parental sequence was determined. Secondly, differential amplification of AT-rich viral variants was obtained by using a denaturation temperature 1–3 °C lower than Tp. Application of this sensitive method to two different viruses allowed us to identify human immunodeficiency virus type 1 G
A hypermutants in a situation where none were expected and to amplify AT-rich variants selectively within a spectrum of poliovirus mutants.
This article has been cited by other articles:
|
|
 |
|
  J. Zielonka, I. G. Bravo, D. Marino, E. Conrad, M. Perkovic, M. Battenberg, K. Cichutek, and C. Munk Restriction of Equine Infectious Anemia Virus by Equine APOBEC3 Cytidine Deaminases J. Virol., August 1, 2009; 83(15): 7547 - 7559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Petit, J.-P. Vartanian, and S. Wain-Hobson Powerful mutators lurking in the genome Phil Trans R Soc B, March 12, 2009; 364(1517): 705 - 715. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Suspene, M. Renard, M. Henry, D. Guetard, D. Puyraimond-Zemmour, A. Billecocq, M. Bouloy, F. Tangy, J.-P. Vartanian, and S. Wain-Hobson Inversing the natural hydrogen bonding rule to selectively amplify GC-rich ADAR-edited RNAs Nucleic Acids Res., July 1, 2008; 36(12): e72 - e72. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kock and H. E. Blum Hypermutation of hepatitis B virus genomes by APOBEC3G, APOBEC3C and APOBEC3H J. Gen. Virol., May 1, 2008; 89(5): 1184 - 1191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-P. Vartanian, D. Guetard, M. Henry, and S. Wain-Hobson Evidence for Editing of Human Papillomavirus DNA by APOBEC3 in Benign and Precancerous Lesions Science, April 11, 2008; 320(5873): 230 - 233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bonvin and J. Greeve Effects of point mutations in the cytidine deaminase domains of APOBEC3B on replication and hypermutation of hepatitis B virus in vitro J. Gen. Virol., December 1, 2007; 88(12): 3270 - 3274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Noguchi, N. Hiraga, N. Mori, M. Tsuge, M. Imamura, S. Takahashi, Y. Fujimoto, H. Ochi, H. Abe, T. Maekawa, et al. Dual effect of APOBEC3G on Hepatitis B virus J. Gen. Virol., February 1, 2007; 88(2): 432 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-L. Chiu and W. C. Greene APOBEC3 Cytidine Deaminases: Distinct Antiviral Actions along the Retroviral Life Cycle J. Biol. Chem., March 31, 2006; 281(13): 8309 - 8312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Delebecque, R. Suspene, S. Calattini, N. Casartelli, A. Saib, A. Froment, S. Wain-Hobson, A. Gessain, J.-P. Vartanian, and O. Schwartz Restriction of Foamy Viruses by APOBEC Cytidine Deaminases J. Virol., January 15, 2006; 80(2): 605 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mahieux, R. Suspene, F. Delebecque, M. Henry, O. Schwartz, S. Wain-Hobson, and J.-P. Vartanian Extensive editing of a small fraction of human T-cell leukemia virus type 1 genomes by four APOBEC3 cytidine deaminases J. Gen. Virol., September 1, 2005; 86(9): 2489 - 2494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Suspene, D. Guetard, M. Henry, P. Sommer, S. Wain-Hobson, and J.-P. Vartanian Extensive editing of both hepatitis B virus DNA strands by APOBEC3 cytidine deaminases in vitro and in vivo PNAS, June 7, 2005; 102(23): 8321 - 8326. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
