HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lee, C.-K. Articles by Tracy, S. Search for Related Content PubMed PubMed Citation Articles by Lee, C.-K. Articles by Tracy, S. Agricola Articles by Lee, C.-K. Articles by Tracy, S.

Characterization of an infectious cDNA copy of the genome of a naturally occurring, avirulent coxsackievirus B3 clinical isolate

C.-K. Lee^1,,

K. Kono^1,

¹ Enterovirus Research Laboratory, Department of Pathology and Microbiology, University of Nebraska Medical Center, 986495 Nebraska Medical Center, Omaha, NE 68198, USA
² Department of Pathology and Microbiology, University of Nebraska Medical Center, 986495 Nebraska Medical Center, Omaha, NE 68198, USA
³ Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, NE 68178, USA

Correspondence
S. Tracy
stracy{at}unmc.edu

Group B coxsackieviruses (CVB) cause numerous diseases, including myocarditis, pancreatitis, aseptic meningitis and possibly type 1 diabetes. To date, infectious cDNA copies of CVB type 3 (CVB3) genomes have all been derived from pathogenic virus strains. An infectious cDNA copy of the well-characterized, non-pathogenic CVB3 strain GA genome was cloned in order to facilitate mapping of the CVB genes that influence expression of a virulence phenotype. Comparison of the sequence of the parental CVB3/GA population, derived by direct RT-PCR-mediated sequence analysis, to that of the infectious CVB3/GA progeny genome demonstrated that an authentic copy was cloned; numerous differences were observed in coding and non-coding sequences relative to other CVB3 strains. Progeny CVB3/GA replicated similarly to the parental strain in three different cell cultures and was avirulent when inoculated into mice, causing neither pancreatitis nor myocarditis. Inoculation of mice with CVB3/GA protected mice completely against myocarditis and pancreatitis induced by cardiovirulent CVB3 challenge. The secondary structure predicted for the CVB3/GA domain II, a region within the 5' non-translated region that is implicated as a key site affecting the expression of a cardiovirulent phenotype, differs from those predicted for cardiovirulent and pancreovirulent CVB3 strains. This is the first report characterizing a cloned CVB3 genome from an avirulent strain.

These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession number for the sequence reported in this paper is AY673831.

Present address: Department of Laboratory Medicine, Korea University Guro Hospital, Guro-gu Guro-dong 80, 152-050 Seoul, Republic of Korea.

This article has been cited by other articles:

				R. Feuer, C. M. Ruller, N. An, J. M. Tabor-Godwin, R. E. Rhoades, S. Maciejewski, R. R. Pagarigan, C. T. Cornell, S. J. Crocker, W. B. Kiosses, et al. Viral Persistence and Chronic Immunopathology in the Adult Central Nervous System following Coxsackievirus Infection during the Neonatal Period J. Virol., September 15, 2009; 83(18): 9356 - 9369. [Abstract] [Full Text] [PDF]

				K.-S. Kim, N. M. Chapman, and S. Tracy Replication of Coxsackievirus B3 in Primary Cell Cultures Generates Novel Viral Genome Deletions J. Virol., February 15, 2008; 82(4): 2033 - 2037. [Abstract] [Full Text] [PDF]

				X. Si, Y. Wang, J. Wong, J. Zhang, B. M. McManus, and H. Luo Dysregulation of the Ubiquitin-Proteasome System by Curcumin Suppresses Coxsackievirus B3 Replication J. Virol., April 1, 2007; 81(7): 3142 - 3150. [Abstract] [Full Text] [PDF]

				J. M. Bailey and W. E. Tapprich Structure of the 5' Nontranslated Region of the Coxsackievirus B3 Genome: Chemical Modification and Comparative Sequence Analysis J. Virol., January 15, 2007; 81(2): 650 - 668. [Abstract] [Full Text] [PDF]

				T. Kanno, K. Kim, K. Kono, K. M. Drescher, N. M. Chapman, and S. Tracy Group B coxsackievirus diabetogenic phenotype correlates with replication efficiency. J. Virol., June 1, 2006; 80(11): 5637 - 5643. [Abstract] [Full Text] [PDF]