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Nucleolar localization of potato leafroll virus capsid proteins

Sophie Haupt^1,2,

Tanya Stroganova^1,

¹ Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK
² University of Dundee, Dundee DD1 4NH, UK
³ University of Warwick – HRI, Wellesbourne, Warwick CV35 9EF, UK

Correspondence
Michael Taliansky
mtalia{at}scri.sari.ac.uk

Potato leafroll virus (PLRV) encodes two capsid proteins, major protein (CP) and minor protein (P5), an extended version of the CP produced by occasional translational ‘readthrough’ of the CP gene. Immunogold electron microscopy showed that PLRV CP is located in the cytoplasm and also localized in the nucleus, preferentially targeting the nucleolus. The nucleolar localization of PLRV CP was also confirmed when it was expressed as a fusion with green fluorescent protein (GFP) via an Agrobacterium vector. Mutational analysis identified a particular sequence within PLRV CP involved in nucleolar targeting [the nucleolar localization signal (NoLS)]. Minor protein P5 also contains the same NoLS, and was targeted to the nucleolus when it was expressed as a fusion with GFP from Agrobacterium. However, P5–GFP lost its nucleolar localization in the presence of replicating PLRV.

These authors contributed equally to this paper.

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				S. H. Kim, S. MacFarlane, N. O. Kalinina, D. V. Rakitina, E. V. Ryabov, T. Gillespie, S. Haupt, J. W. S. Brown, and M. Taliansky Interaction of a plant virus-encoded protein with the major nucleolar protein fibrillarin is required for systemic virus infection PNAS, June 26, 2007; 104(26): 11115 - 11120. [Abstract] [Full Text] [PDF]