| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Short Communication |
1 Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Level 5 Box 157, Hills Road, Cambridge CB2 2QQ, UK
2 Cambridge Institute for Medical Research, School of Clinical Medicine, University of Cambridge, Addenbrooke's Hospital, Level 5 Box 157, Hills Road, Cambridge CB2 2QQ, UK
Correspondence
J. H. Sinclair
js{at}mole.bio.cam.ac.uk
Human cytomegalovirus (HCMV) is a frequent cause of major disease following primary infection or reactivation from latency in immunocompromised patients. Infection of non-permissive mononuclear cells is used for analyses of HCMV latency in vitro. Using this approach, it is shown here that repression of lytic gene expression following experimental infection of CD34+ cells, a site of HCMV latency in vivo, correlates with recruitment of repressive chromatin around the major immediate-early promoter (MIEP). Furthermore, long-term culture of CD34+ cells results in carriage of viral genomes in which the MIEP remains associated with transcriptionally repressive chromatin. Finally, specific differentiation of long-term cultures of infected CD34+ cells to mature dendritic cells results in acetylation of histones bound to the MIEP, concomitant loss of heterochromatin protein 1 and the reactivation of HCMV. These data are consistent with ex vivo analyses of latency and may provide a model for further analyses of the mechanisms involved during latency and reactivation.
Published online ahead of print on 31 August 2005 as DOI 10.1099/vir.0.81161-0.
This article has been cited by other articles:
|
|
 |
|
  J. Yuan, X. Liu, A. W. Wu, P. W. McGonagill, M. J. Keller, C. S. Galle, and J. L. Meier Breaking Human Cytomegalovirus Major Immediate-Early Gene Silence by Vasoactive Intestinal Peptide Stimulation of the Protein Kinase A-CREB-TORC2 Signaling Cascade in Human Pluripotent Embryonal NTera2 Cells J. Virol., July 1, 2009; 83(13): 6391 - 6403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Petrucelli, M. Rak, L. Grainger, and F. Goodrum Characterization of a Novel Golgi Apparatus-Localized Latency Determinant Encoded by Human Cytomegalovirus J. Virol., June 1, 2009; 83(11): 5615 - 5629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Crough and R. Khanna Immunobiology of Human Cytomegalovirus: from Bench to Bedside Clin. Microbiol. Rev., January 1, 2009; 22(1): 76 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Sinzger, G. Hahn, M. Digel, R. Katona, K. L. Sampaio, M. Messerle, H. Hengel, U. Koszinowski, W. Brune, and B. Adler Cloning and sequencing of a highly productive, endotheliotropic virus strain derived from human cytomegalovirus TB40/E J. Gen. Virol., February 1, 2008; 89(2): 359 - 368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. J. Groves and J. H. Sinclair Knockdown of hDaxx in normally non-permissive undifferentiated cells does not permit human cytomegalovirus immediate-early gene expression J. Gen. Virol., November 1, 2007; 88(11): 2935 - 2940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Saffert and R. F. Kalejta Human Cytomegalovirus Gene Expression Is Silenced by Daxx-Mediated Intrinsic Immune Defense in Model Latent Infections Established In Vitro J. Virol., September 1, 2007; 81(17): 9109 - 9120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Keller, A. W. Wu, J. I. Andrews, P. W. McGonagill, E. E. Tibesar, and J. L. Meier Reversal of Human Cytomegalovirus Major Immediate-Early Enhancer/Promoter Silencing in Quiescently Infected Cells via the Cyclic AMP Signaling Pathway J. Virol., June 15, 2007; 81(12): 6669 - 6681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Woodhall, I. J. Groves, M. B. Reeves, G. Wilkinson, and J. H. Sinclair Human Daxx-mediated Repression of Human Cytomegalovirus Gene Expression Correlates with a Repressive Chromatin Structure around the Major Immediate Early Promoter J. Biol. Chem., December 8, 2006; 281(49): 37652 - 37660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. L. Cheung, A. Abendroth, A. L. Cunningham, and B. Slobedman Viral gene expression during the establishment of human cytomegalovirus latent infection in myeloid progenitor cells Blood, December 1, 2006; 108(12): 3691 - 3699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Tavalai, P. Papior, S. Rechter, M. Leis, and T. Stamminger Evidence for a Role of the Cellular ND10 Protein PML in Mediating Intrinsic Immunity against Human Cytomegalovirus Infections. J. Virol., August 1, 2006; 80(16): 8006 - 8018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sinclair and P. Sissons Latency and reactivation of human cytomegalovirus J. Gen. Virol., July 1, 2006; 87(7): 1763 - 1779. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
