| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Short Communication |
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK
Correspondence
Paul Targett-Adams
p.targett-adams{at}vir.gla.ac.uk
Dicistronic, subgenomic hepatitis C virus (HCV) replicons were constructed containing sequences from JFH1, a genotype 2a strain, that also incorporated the firefly luciferase gene under the control of the HCV internal ribosome entry site element. Luciferase activity in Huh-7 cell extracts containing in vitro-transcribed subgenomic JFH1 RNA was monitored over a 72 h period to examine early stages of HCV replication in the absence of any selective pressure. Enzyme activities produced by the replicon were almost 200-fold greater than those generated from corresponding genotype 1b replicons and correlated with an accumulation of NS5A protein and replicon RNA. Transient replication was sensitive to IFN treatment in a dose-dependent manner and, in addition to Huh-7 cells, the U2OS human osteosarcoma cell line supported efficient replication of the JFH1 replicon. Thus, this system based on JFH1 sequences offers improvements over prior genotype 1b replicons for quantitative measurement of viral RNA replication.
Quantitative RT-PCR results are available as supplementary material in JGV Online.
This article has been cited by other articles:
|
|
 |
|
  M. Hughes, S. Gretton, H. Shelton, D. D. Brown, C. J. McCormick, A. G. N. Angus, A. H. Patel, S. Griffin, and M. Harris A Conserved Proline between Domains II and III of Hepatitis C Virus NS5A Influences both RNA Replication and Virus Assembly J. Virol., October 15, 2009; 83(20): 10788 - 10796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Roohvand, P. Maillard, J.-P. Lavergne, S. Boulant, M. Walic, U. Andreo, L. Goueslain, F. Helle, A. Mallet, J. McLauchlan, et al. Initiation of Hepatitis C Virus Infection Requires the Dynamic Microtubule Network: ROLE OF THE VIRAL NUCLEOCAPSID PROTEIN J. Biol. Chem., May 15, 2009; 284(20): 13778 - 13791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Jones, A. H. Patel, P. Targett-Adams, and J. McLauchlan The Hepatitis C Virus NS4B Protein Can trans-Complement Viral RNA Replication and Modulates Production of Infectious Virus J. Virol., March 1, 2009; 83(5): 2163 - 2177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ratinier, S. Boulant, C. Combet, P. Targett-Adams, J. McLauchlan, and J.-P. Lavergne Transcriptional slippage prompts recoding in alternate reading frames in the hepatitis C virus (HCV) core sequence from strain HCV-1 J. Gen. Virol., July 1, 2008; 89(7): 1569 - 1578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Targett-Adams, S. Boulant, and J. McLauchlan Visualization of Double-Stranded RNA in Cells Supporting Hepatitis C Virus RNA Replication J. Virol., March 1, 2008; 82(5): 2182 - 2195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Binder, D. Quinkert, O. Bochkarova, R. Klein, N. Kezmic, R. Bartenschlager, and V. Lohmann Identification of Determinants Involved in Initiation of Hepatitis C Virus RNA Synthesis by Using Intergenotypic Replicase Chimeras J. Virol., May 15, 2007; 81(10): 5270 - 5283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Jones, S. N. Gretton, J. McLauchlan, and P. Targett-Adams Mobility analysis of an NS5A-GFP fusion protein in cells actively replicating hepatitis C virus subgenomic RNA J. Gen. Virol., February 1, 2007; 88(2): 470 - 475. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
