HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary tables Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Teycheney, P.-Y. Articles by Candresse, T. Search for Related Content PubMed PubMed Citation Articles by Teycheney, P.-Y. Articles by Candresse, T. Agricola Articles by Teycheney, P.-Y. Articles by Candresse, T.

High genetic variability and evidence for plant-to-plant transfer of Banana mild mosaic virus

¹ CIRAD, UPR 75, Station de Neufchâteau, Sainte-Marie, F-97130 Capesterre Belle-Eau, Guadeloupe, French West Indies
² CIRAD/UMR BGPI, TA 41/K, Campus International de Baillarguet, F-34398 Montpellier Cedex, France
³ UMR GD2P, INRA et Université Bordeaux 2, IBVM, Campus INRA de la Grande Ferrade, BP 81, F-33883 Villenave d'Ornon Cedex, France

Correspondence
Pierre-Yves Teycheney
teycheney{at}cirad.fr

A total of 154 partial nucleotide sequences within the Banana mild mosaic virus (BanMMV) ORF1, which encodes the viral RNA-dependent RNA polymerase (RdRp), was obtained from 68 distinct infected banana accessions originating from various locations worldwide. The 310 nt sequences displayed a high level of variability with a mean pairwise nucleotide sequence divergence level of 20·4 %. This situation resulted essentially from a high rate of synonymous mutations. A similar analysis was performed for a limited selection of 10 banana accessions (30 sequences) on the region comprising approximately the last 310 nt of the BanMMV genome. This region corresponds to the 3' end of ORF5, which encodes the coat protein (234 nt), and to the 3' non-coding region. This analysis confirmed the high level of diversity observed in the RdRp dataset, characterized by a high level of synonymous mutations. Analysis of intra-host diversity indicated the existence of two distinct situations, with some plants containing only closely related sequence variants, whereas others contained widely divergent isolates. Analyses indicated that BanMMV genetic diversity is not structured by the geographical origin of the infected Musa accessions or by their genotype. This situation may be, in part, explained by the exchange of banana germplasm between different parts of the world and also by plant-to-plant transfer of virus isolates, the evidence for which is, for the first time, provided by this study.

The GenBank/EMBL/DDBJ accession numbers of the sequences reported in this paper are AY729491–AY729643 (RdRp sequences) and AY730729–AY730758 (CP/3' NCR sequences).

Tables showing the names, genomic groups, origins and sequences generated from banana accessions are available as supplementary material in JGV Online.

This article has been cited by other articles:

				S. Martin, M. L. Garcia, A. Troisi, L. Rubio, G. Legarreta, O. Grau, D. Alioto, P. Moreno, and J. Guerri Genetic variation of populations of Citrus psorosis virus. J. Gen. Virol., October 1, 2006; 87(Pt 10): 3097 - 3102. [Abstract] [Full Text] [PDF]

				B. Meng, A. R. Rebelo, and H. Fisher Genetic diversity analyses of grapevine Rupestris stem pitting-associated virus reveal distinct population structures in scion versus rootstock varieties J. Gen. Virol., June 1, 2006; 87(6): 1725 - 1733. [Abstract] [Full Text] [PDF]