HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Revill, P. Articles by Harding, R. Search for Related Content PubMed PubMed Citation Articles by Revill, P. Articles by Harding, R. Agricola Articles by Revill, P. Articles by Harding, R.

Taro vein chlorosis virus: characterization and variability of a new nucleorhabdovirus

Peter Revill

Science Research Centre, Queensland University of Technology, GPO Box 2434, Brisbane, Queensland 4001, Australia

Correspondence
Rob Harding
r.harding{at}qut.edu.au

Sequencing of the monopartite RNA genome of a Fijian isolate of Taro vein chlorosis virus (TaVCV) confirmed that it is a definitive rhabdovirus with most similarity to members of the genus Nucleorhabdovirus. The TaVCV 12 020 nt negative-sense RNA genome contained six ORFs in the antigenomic sequence, equivalent to the N, P, 3, M, G and L genes that have been identified in other rhabdoviruses. The putative gene products had highest similarity to those of the nucleorhabdovirus Maize mosaic virus. A characteristic 3'-AAUUCUUUUUGGGUUGU/A-5' sequence was identified in each of the intergenic regions and the TaVCV leader and trailer sequences comprised 140 and 61 nt, respectively. Assignment of TaVCV to the genus Nucleorhabdovirus was supported by thin-section electron microscopy of TaVCV-infected taro leaves, which identified virions budding from nuclear membranes into the perinuclear space. Variability studies identified high levels of TaVCV sequence diversity. Within the L gene of 20 TaVCV isolates from Fiji, the Federated States of Micronesia, New Caledonia, Papua New Guinea, Solomon Islands and Vanuatu, maximum variability at the nucleotide level was 27·4 %. Within the N gene, maximum variability among 15 isolates at the nucleotide level was 19·3 %. The high level of TaVCV variability observed suggested that the introduction of TaVCV to the Pacific Islands was not a recent occurrence.

The GenBank/EMBL/DDBJ accession number for the sequence reported in this paper is AY674964.

Present address: Victorian Infectious Diseases Reference Laboratory, 10 Wreckyn St, Nth Melbourne, Victoria, Australia.

This article has been cited by other articles:

				M. M. Goodin, R. Chakrabarty, S. Yelton, K. Martin, A. Clark, and R. Brooks Membrane and protein dynamics in live plant nuclei infected with Sonchus yellow net virus, a plant-adapted rhabdovirus J. Gen. Virol., June 1, 2007; 88(6): 1810 - 1820. [Abstract] [Full Text] [PDF]

				S. Martin, M. L. Garcia, A. Troisi, L. Rubio, G. Legarreta, O. Grau, D. Alioto, P. Moreno, and J. Guerri Genetic variation of populations of Citrus psorosis virus. J. Gen. Virol., October 1, 2006; 87(Pt 10): 3097 - 3102. [Abstract] [Full Text] [PDF]

				H. Kondo, T. Maeda, Y. Shirako, and T. Tamada Orchid fleck virus is a rhabdovirus with an unusual bipartite genome. J. Gen. Virol., August 1, 2006; 87(Pt 8): 2413 - 2421. [Abstract] [Full Text] [PDF]