J Gen Virol
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J Gen Virol 86 (2005), 789-796; DOI 10.1099/vir.0.80543-0

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© 2005 Society for General Microbiology

Isolation and identification of a new tetravirus from Dendrolimus punctatus larvae collected from Yunnan Province, China

Fuming Yi, Jiamin Zhang, Haiyang Yu, Chuanfeng Liu, Junping Wang and Yuanyang Hu

Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China

Correspondence
Yuanyang Hu
whuhyy{at}163.com

In this study, Dendrolimus punctatus tetravirus (DpTV) has been identified as a new member of the genus Omegatetravirus of the family Tetraviridae that may be related serologically to Nudaurelia capensis {omega} virus (N{omega}V). DpTV particles are isometric, with a diameter of about 40 nm and a buoyant density of 1·281 g cm–3 in CsCl. The virus has two capsid proteins (of 62 500 and 6800 Da) and two single-stranded RNA molecules (RNA1 and RNA2), which are 5492 and 2490 nt long, respectively. RNA1 has a large open reading frame (ORF) encoding a polypeptide of 180 kDa; RNA2 contains two partially overlapping ORFs encoding polypeptides of 17 and 70 kDa. The 180 kDa protein, which contains consensus motifs of a putative methyltransferase, helicase and RNA-dependent RNA polymerase, shows significant similarity to those of other tetraviruses. The 17 kDa protein is a PEST (Pro/Glu/Ser/Thr) protein of unknown function. The 70 kDa protein is the coat protein precursor and is predicted to be cleaved at an Asn–Phe site located after residue 570. The 70 kDa protein shows 86 and 66 % identity to its homologues in N{omega}V and Helicoverpa armigera stunt virus, respectively. Secondary-structure analysis revealed that the RNAs of DpTV have tRNA-like structures at their 3' termini.

The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences reported in this article are AY594352 (RNA1) and AY594353 (RNA2).




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