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Minute virus of mice small non-structural protein NS2 localizes within, but is not required for the formation of, Smn-associated autonomous parvovirus-associated replication bodies

Philip J. Young^1,

Ann Newman^2,

¹ Department of Veterinary Pathobiology, Life Sciences Center 471G, University of Missouri, Columbia, MO 65211-7310, USA
² Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65212, USA
³ Novozymes, 1AS.31, Novo Alle, DK-2880 Bagsværd, Denmark

Correspondence
Christian L. Lorson
lorsonc{at}missouri.edu

The non-structural proteins NS1 and NS2 of the parvovirus minute virus of mice (MVM) are required for efficient virus replication. It has previously been shown that NS1 and NS2 interact and colocalize with the survival motor neuron (Smn) gene product in novel nuclear structures that are formed late in infection, termed Smn-associated APAR (autonomous parvovirus-associated replication) bodies (SAABs). It is not clear what molecular viral intermediate(s) contribute to SAAB formation. The current results address the role of NS2 in SAAB formation. In highly synchronized wild-type MVM infection of murine A9_2L cells, NS2 colocalizes with Smn and other SAAB constituents. An MVM mutant that does not produce NS2 still generates SAABS, albeit with a temporal delay. The lag in SAAB formation seen in the absence of NS2 is probably related to the temporal delay in virus replication, suggesting that, whilst NS2 is required for efficient viral infection, it is dispensable for SAAB formation.

These authors contributed equally to this work.

This article has been cited by other articles:

				S. F. Cotmore, R. L. Gottlieb, and P. Tattersall Replication Initiator Protein NS1 of the Parvovirus Minute Virus of Mice Binds to Modular Divergent Sites Distributed throughout Duplex Viral DNA J. Virol., December 1, 2007; 81(23): 13015 - 13027. [Abstract] [Full Text] [PDF]