| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Molecular Virology Laboratory, Hellenic Pasteur Institute, 127 Vas. Sofias Avenue, Athens 115 21, Greece
Correspondence
Penelope Mavromara
penelopm{at}hol.gr
Translation of the hepatitis C virus (HCV) polyprotein is mediated by an internal ribosome entry site (IRES) that is located mainly within the 5' non-translated region of the viral genome. In this study, the effect of the HCV non-structural 5A (NS5A) protein on the HCV IRES-dependent translation was investigated by using a transient transfection system. Three different cell lines (HepG2, WRL-68 and BHK-21) were co-transfected with a plasmid vector containing a bicistronic transcript carrying the chloramphenicol acetyltransferase (CAT) and the firefly luciferase genes separated by the HCV IRES sequences, and an expression vector producing the NS5A protein. Here, it was shown that the HCV NS5A protein inhibited HCV IRES-dependent translation in a dose-dependent manner. In contrast, NS5A had no detectable effect on cap-dependent translation of the upstream gene (CAT) nor on translation from another viral IRES. Further analysis using deleted forms of the NS5A protein revealed that a region of about 120 aa located just upstream of the nuclear localization signal of the protein is critical for this suppression. Overall, these results suggest that HCV NS5A protein negatively modulates the HCV IRES activity in a specific manner.
Published online ahead of print on 17 January 2005 as DOI 10.1099/vir.0.80728-0
This article has been cited by other articles:
|
|
 |
|
  L. Jaffrelo, S. Chabas, S. Reigadas, A. Pflieger, C. Wychowski, J. Rumi, M. Ventura, J.-J. Toulme, and C. Staedel A functional selection of viral genetic elements in cultured cells to identify hepatitis C virus RNA translation inhibitors Nucleic Acids Res., September 1, 2008; 36(15): e95 - e95. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Xiao, Y. Bai, H. Xu, X. Geng, J. Chen, Y. Wang, J. Chen, and B. Li Effect of NS3 and NS5B proteins on classical swine fever virus internal ribosome entry site-mediated translation and its host cellular translation J. Gen. Virol., April 1, 2008; 89(4): 994 - 999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Masante, K. Mahias, S. Lourenco, E. Dumas, A. Cahour, P. Trimoulet, H. Fleury, T. Astier-Gin, and M. Ventura Seven nucleotide changes characteristic of the hepatitis C virus genotype 3 5' untranslated region: correlation with reduced in vitro replication J. Gen. Virol., January 1, 2008; 89(1): 212 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Isken, M. Baroth, C. W. Grassmann, S. Weinlich, D. H. Ostareck, A. Ostareck-Lederer, and S.-E. Behrens Nuclear factors are involved in hepatitis C virus RNA replication RNA, October 1, 2007; 13(10): 1675 - 1692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. MacCallum, S. C. Jack, P. A. Egan, B. T. McDermott, R. M. Elliott, and S.-W. Chan Cap-dependent and hepatitis C virus internal ribosome entry site-mediated translation are modulated by phosphorylation of eIF2{alpha} under oxidative stress. J. Gen. Virol., November 1, 2006; 87(Pt 11): 3251 - 3262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kalamvoki, U. Georgopoulou, and P. Mavromara The NS5A Protein of the Hepatitis C Virus Genotype 1a Is Cleaved by Caspases to Produce C-terminal-truncated Forms of the Protein That Reside Mainly in the Cytosol J. Biol. Chem., May 12, 2006; 281(19): 13449 - 13462. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
