J Gen Virol
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J Gen Virol 86 (2005), 929-943; DOI 10.1099/vir.0.80490-0

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© 2005 Society for General Microbiology

Analysis of the Choristoneura fumiferana nucleopolyhedrovirus genome

Jondavid G. de Jong1, Hilary A. M. Lauzon2, Cliff Dominy1, Arkadi Poloumienko1, Eric B. Carstens3, Basil M. Arif2 and Peter J. Krell1

1 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
2 Great Lakes Forestry Centre, Sault Ste Marie, Ontario, Canada, P6A 2E5
3 Department of Microbiology and Immunology, Queens University, Kingston, Ontario, Canada, K7L 3N6

Correspondence
Peter J. Krell
pkrell{at}uoguelph.ca

The double-stranded DNA genome of Choristoneura fumiferana nucleopolyhedrovirus (CfMNPV) was sequenced and analysed in the context of other group I nucleopolyhedroviruses (NPVs). The genome consists of 129 593 bp with a G+C content of 50·1 mol%. A total of 146 open reading frames (ORFs) of greater than 150 bp, and with no or minimal overlap were identified. In addition, five homologous regions were identified containing 7–10 repeats of a 36 bp imperfect palindromic core. Comparison with other completely sequenced baculovirus genomes revealed that 139 of the CfMNPV ORFs have homologues in at least one other baculovirus and seven ORFs are unique to CfMNPV. Of the 117 CfMNPV ORFs common to all group I NPVs, 12 are exclusive to group I NPVs. Overall, CfMNPV is most similar to Orgyia pseudotsugata MNPV based on gene content, arrangement and overall amino acid identity. Unlike other group I baculoviruses, however, CfMNPV encodes a viral enhancing factor (vef) and has two copies of p26.

The GenBank/EMBL/DDBJ accession number of the complete genome sequence of CfMNPV reported in this paper is AF512031.




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