HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Huang, R. Articles by Shi, Z. Search for Related Content PubMed PubMed Citation Articles by Huang, R. Articles by Shi, Z. Agricola Articles by Huang, R. Articles by Shi, Z.

A novel envelope protein involved in White spot syndrome virus infection

State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071 Wuhan, People's Republic of China

Correspondence
Zhengli Shi
zlshi{at}pentium.whiov.ac.cn

One open reading frame (designated vp76) from the White spot syndrome virus (WSSV) genome has the motif of a cytokine I receptor and has been identified as a structural protein. In this paper, vp76 was expressed in Escherichia coli and used to prepare a specific antibody to determine the location of the corresponding protein in the intact virion, the nucleocapsids and the envelope of WSSV. Western blotting with the VP76 antiserum confirmed that VP76 was an envelope protein of WSSV. To investigate the function of the VP76, WSSV was neutralized with the VP76-specific antiserum at different concentrations and injected intramuscularly into crayfish. The mortality curves showed that the VP76 antiserum could partially attenuate infection with WSSV, suggesting that VP76 is an envelope protein involved in WSSV infection.

This article has been cited by other articles:

				Z. Li, Q. Lin, J. Chen, J. L. Wu, T. K. Lim, S. S. Loh, X. Tang, and C.-L. Hew Shotgun Identification of the Structural Proteome of Shrimp White Spot Syndrome Virus and iTRAQ Differentiation of Envelope and Nucleocapsid Subproteomes Mol. Cell. Proteomics, September 1, 2007; 6(9): 1609 - 1620. [Abstract] [Full Text] [PDF]

				X. Xie, L. Xu, and F. Yang Proteomic Analysis of the Major Envelope and Nucleocapsid Proteins of White Spot Syndrome Virus J. Virol., November 1, 2006; 80(21): 10615 - 10623. [Abstract] [Full Text] [PDF]

				J.-M. Tsai, H.-C. Wang, J.-H. Leu, A. H.-J. Wang, Y. Zhuang, P. J. Walker, G.-H. Kou, and C.-F. Lo Identification of the Nucleocapsid, Tegument, and Envelope Proteins of the Shrimp White Spot Syndrome Virus Virion J. Virol., March 15, 2006; 80(6): 3021 - 3029. [Abstract] [Full Text] [PDF]