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J Gen Virol 86 (2005), 1415-1421; DOI 10.1099/vir.0.80768-0

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© 2005 Society for General Microbiology

Short Communication

Mobility of the hepatitis C virus NS4B protein on the endoplasmic reticulum membrane and membrane-associated foci

Sarah N. Gretton{dagger}, Annette I. Taylor{dagger},{ddagger} and John McLauchlan

MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK

Correspondence
John McLauchlan
j.mclauchlan{at}vir.gla.ac.uk

The hepatitis C virus (HCV) non-structural protein NS4B induces morphological changes in the endoplasmic reticulum (ER) membrane that may have a direct role in viral RNA replication. A chimeric GFP–NS4B fusion protein located to the ER membrane and to foci that were attached to the ER. These membrane-associated foci (MAFs) could be related to the membrane alterations observed in cells that replicate HCV RNA. The relationship of MAFs to pre-existing cellular structures is not known. Indirect immunofluorescence analysis demonstrated that they did not contain a cellular marker for vesicles, which have been implicated in the replication of other viruses. From photobleaching studies to examine diffusion of NS4B, the GFP-tagged protein had reduced mobility on MAFs compared with on the ER membrane. This slower mobility suggested that NS4B is likely to form different interactions on MAFs and the ER.

{dagger}These authors contributed equally to this work.

{ddagger}Present address: Ingenza Ltd, King's Buildings, Edinburgh EH9 3JJ, UK.




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