HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary figures Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shirasawa-Seo, N. Articles by Matsumoto, T. Search for Related Content PubMed PubMed Citation Articles by Shirasawa-Seo, N. Articles by Matsumoto, T. Agricola Articles by Shirasawa-Seo, N. Articles by Matsumoto, T.

Characteristics of the promoters derived from the single-stranded DNA components of Milk vetch dwarf virus in transgenic tobacco

¹ Miyagi Prefectural Agriculture and Horticulture Research Center, Takadate-kawakami, Natori, Miyagi 981-1243, Japan
² Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan
³ Laboratory of Plant Pathology, Faculty of Agriculture, Niigata University, Ikarashi, Niigata 950-2181, Japan
⁴ National Institute of Agro-Biological Sciences, Kannondai, Tsukuba, Ibaraki 305-8602, Japan

Correspondence
Shigeo Nakamura
nakamura-sh894{at}pref.miyagi.jp

Predicted promoter regions of Milk vetch dwarf virus (MDV) components (C1–C11) were isolated and fused with a -glucuronidase (GUS) reporter gene and the characteristics of the promoters were examined. In transgenic tobacco calli, promoters of MDV C4 (encoding a cell-cycle link protein), C5 and C7 (both encoding unknown proteins), C6 (encoding a nuclear-shuttle protein) and C8 (encoding a movement protein) generated a stronger level of GUS expression than the Cauliflower mosaic virus 35S RNA promoter (P35S). In leaves of transgenic tobacco plants, the promoters of C5 and C8 conferred a level of GUS activity comparable to that of P35S. Histochemical GUS analysis showed that the promoters of C4–C9, the latter encoding a capsid protein, were active in phloem and meristematic tissue. The promoter of C8 was also active in mesophyll and cortex cell types. A low level of activity was found for the promoters of C11, which encodes a master replication-initiator protein (Rep), and C1, C2, C3 and C10, which encode additional Reps, in both transgenic tobacco calli and plants.

Graphs showing transient GUS expression of MDV-derived promoters in pea and tobacco leaves (Supplementary Fig. S1) and GUS expression by MDV-derived promoters in E. coli and A. tumefaciens (Supplementary Fig. S2) are available as supplementary material in JGV Online.

This article has been cited by other articles:

				I. Grigoras, T. Timchenko, and B. Gronenborn Transcripts encoding the nanovirus master replication initiator proteins are terminally redundant J. Gen. Virol., February 1, 2008; 89(2): 583 - 593. [Abstract] [Full Text] [PDF]

				S. Lageix, O. Catrice, J.-M. Deragon, B. Gronenborn, T. Pelissier, and B. C. Ramirez The Nanovirus-Encoded Clink Protein Affects Plant Cell Cycle Regulation through Interaction with the Retinoblastoma-Related Protein J. Virol., April 15, 2007; 81(8): 4177 - 4185. [Abstract] [Full Text] [PDF]

				T. Timchenko, L. Katul, M. Aronson, J. C. Vega-Arreguin, B. C. Ramirez, H. J. Vetten, and B. Gronenborn Infectivity of nanovirus DNAs: induction of disease by cloned genome components of Faba bean necrotic yellows virus J. Gen. Virol., June 1, 2006; 87(6): 1735 - 1743. [Abstract] [Full Text] [PDF]