| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln, NE 68583, USA
Correspondence
C. Jones
cjones{at}unlnotes.unl.edu
Bovine herpesvirus 1 (BHV-1) infected-cell protein 0 (bICP0) stimulates productive infection by activating viral gene expression. In this study, an attempt was made to construct a recombinant virus with point mutations in the C3HC4 zinc RING finger of bICP0, as this domain is necessary for activating viral transcription and productive infection. A virus was identified in bovine cells that induced small clusters of infected cells resembling a small plaque. Instead of the expected mutations within the zinc RING finger, this virus contained a point mutation within the initiating ATG of bICP0, a point mutation two bases downstream from the ATG mutation and deletion of flanking plasmid sequences used for homologous recombination. The bICP0 mutant was rescued with wild-type (wt) bICP0 sequences and the bICP0-rescued virus produced wt plaques. The bICP0-rescued virus and wt BHV-1, but not the mutant, expressed the bICP0 protein during productive infection of bovine cells, suggesting that the mutant virus was a null mutant. Consequently, the mutant was designated the bICP0 null mutant. Infection of bovine cells with the bICP0 null mutant resulted in at least 100-fold lower virus titres, indicating that bICP0 protein expression is important, but not required, for virus production. When bovine cells infected with the bICP0 null mutant virus were subcultured, the cells continued to divide, but viral DNA could be detected after more than 35 passages, suggesting that the bICP0 null mutant induced a persistent-like infection in bovine cells and that it may be useful for generating additional bICP0 mutants.
This article has been cited by other articles:
|
|
 |
|
  K. Saira, S. Chowdhury, N. Gaudreault, L. da Silva, G. Henderson, A. Doster, and C. Jones The Zinc RING Finger of Bovine Herpesvirus 1-Encoded bICP0 Protein Is Crucial for Viral Replication and Virulence J. Virol., December 15, 2008; 82(24): 12060 - 12068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Robinson, J. Meers, J. L. Gravel, F. M. McCarthy, and T. J. Mahony The essential and non-essential genes of Bovine herpesvirus 1 J. Gen. Virol., November 1, 2008; 89(11): 2851 - 2863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, Y. Jiang, V. Geiser, J. Zhou, and C. Jones Bovine herpesvirus 1 immediate-early protein (bICP0) interacts with the histone acetyltransferase p300, which stimulates productive infection and gC promoter activity J. Gen. Virol., July 1, 2006; 87(7): 1843 - 1851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Trapp, M. S. Parcells, J. P. Kamil, D. Schumacher, B. K. Tischer, P. M. Kumar, V. K. Nair, and N. Osterrieder A virus-encoded telomerase RNA promotes malignant T cell lymphomagenesis J. Exp. Med., May 15, 2006; 203(5): 1307 - 1317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Hohle, A. Karger, P. Konig, K. Giesow, and G. M. Keil High-level expression of biologically active bovine alpha interferon by Bovine herpesvirus 1 interferes only marginally with recombinant virus replication in vitro J. Gen. Virol., October 1, 2005; 86(10): 2685 - 2695. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
