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Isolation and cloning of the raccoon (Procyon lotor) papillomavirus type 1 by using degenerate papillomavirus-specific primers

Mads Bertelsen^2,3,

Rolf-Arne Olberg^2,3,

¹ Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium
² Department of Pathobiology, Ontario Veterinary College, University of Guelph, Ontario N1G 2W1, Canada
³ Toronto Zoo, Ontario M1B 5K7, Canada
⁴ The Jackson Laboratory, Bar Harbor, ME 04609-1500, USA

Correspondence
Marc Van Ranst
marc.vanranst{at}uz.kuleuven.ac.be

Partial sequences of a novel papillomavirus were amplified from a cutaneous lesion biopsy of a raccoon (Procyon lotor), by using PCR with degenerate papillomavirus-specific primers. The Procyon lotor papillomavirus type 1 (PlPV-1) DNA was amplified with long template PCR in two overlapping fragments, together encompassing the entire genome, and the complete PlPV-1 genomic sequence was determined. The PlPV-1 genome consists of 8170 bp, and contains the typical papillomaviral open reading frames, encoding five early proteins and two late capsid proteins. Besides the classical non-coding region (NCR1) between the end of L1 and the start of E6, PlPV-1 contains an additional non-coding region (NCR2) of 1065 bp between the early and late protein region, which has previously also been described for the canine oral papillomavirus (COPV) and the Felis domesticus papillomavirus (FdPV-1). Phylogenetic analysis places PlPV-1 together with COPV and FdPV-1 in a monophyletic branch which encompasses the Lambda papillomavirus genus.

The GenBank/EMBL/DDBJ accession number of the sequence reported in this paper is AY763115.

Supplementary material is available in JGV Online.

Present address: Copenhagen Zoo, Copenhagen, Denmark.

Present address: Kristiansand Zoo, Kristiansand, Norway.

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