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J Gen Virol 86 (2005), 2107-2113; DOI 10.1099/vir.0.80958-0

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© 2005 Society for General Microbiology

Phage display-selected single-chain antibodies confer high levels of resistance against Tomato spotted wilt virus

Marcel Prins1, Dick Lohuis1, Arjen Schots2 and Rob Goldbach1

1 Laboratory of Virology, Wageningen University, The Netherlands
2 Laboratory of Molecular Recognition and Antibody Technology, Wageningen University, The Netherlands

Correspondence
Marcel Prins
marcel.prins{at}wur.nl

Rational design of antibodies targeting essential viral proteins can complement the palette of antiviral resistance strategies. Here, stable and high expression of single-chain monoclonal antibodies targeting the nucleoprotein of the economically important plant virus Tomato spotted wilt virus, a protein that is involved in multiple steps in the viral infection cycle, is reported. High cytoplasmic expression levels of three selected phage display-derived anti-viral single-chain antibodies were established. Of these antibodies, two led to high levels of resistance against this plant virus. Protoplast experiments provided evidence that the two resistance-conferring antibodies may have a different mode of action and could be combined for higher durability of resistance in the field.

Fig. 3 is available in colour as supplementary material in JGV Online.




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