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J Gen Virol 87 (2006), 2879-2884; DOI 10.1099/vir.0.81927-0

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© 2006 Society for General Microbiology

Short Communication

Epstein–Barr virus nuclear antigen 3A contains six nuclear-localization signals

Marion Buck1, Anita Burgess1, Roslynn Stirzaker1,2,{dagger}, Kenia Krauer1 and Tom Sculley1

1 Queensland Institute of Medical Research and Griffith Medical Research Centre, Griffith University, 300 Herston Road, Brisbane, QLD 4029, Australia
2 Queensland University of Technology, School of Life Sciences, GPO Box 2434, Brisbane, QLD 4001, Australia

Correspondence
Anita Burgess
Anita.Burgess{at}qimr.edu.au

The Epstein–Barr nuclear antigen 3A (EBNA3A) is one of only six viral proteins essential for Epstein–Barr virus-induced transformation of primary human B cells in vitro. Viral proteins such as EBNA3A are able to interact with cellular proteins, manipulating various biochemical and signalling pathways to initiate and maintain the transformed state of infected cells. EBNA3A has been reported to have one nuclear-localization signal and is targeted to the nucleus during transformation, where it associates with components of the nuclear matrix. By using enhanced green fluorescent protein-tagged deletion mutants of EBNA3A in combination with site-directed mutagenesis, an additional five functional nuclear-localization signals have been identified in the EBNA3A protein. Two of these (aa 63–66 and 375–381) were computer-predicted, whilst the remaining three (aa 394–398, 573–578 and 598–603) were defined functionally in this study.

{dagger}Present address: The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia.






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