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Temporal and differential gene expression of Singapore grouper iridovirus

Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260

Correspondence
Choy Leong Hew
dbshewcl{at}nus.edu.sg
or
dbshead{at}nus.edu.sg

Singapore grouper iridovirus (SGIV), an iridovirus in the genus Ranavirus, is a major pathogen that results in significant economic losses in grouper aquaculture. To investigate further its infective mechanisms, for the first time, a viral DNA microarray was generated for the SGIV genome to measure the expression of its predicted open reading frames simultaneously in vitro. By using the viral DNA microarray, the temporal gene expression of SGIV was characterized and the DNA microarray data were consistent with the results of real-time RT-PCR studies. Furthermore, different-stage viral genes (i.e. immediate-early, early and late genes) of SGIV were uncovered by combining drug treatments and DNA microarray studies. These results should offer important insights into the replication and pathogenesis of iridoviruses.

Supplementary tables showing SGIV primers, partial cDNA sequences of -actin and GAPDH, primers for real-time PCR, and SGIV genes with temporal expression are available in JGV Online.

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