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Genetic variation of populations of Citrus psorosis virus

¹ Instituto Valenciano de Investigaciones Agrarias, Cra Moncada-Náquera Km 4.5, Moncada, 46113 Valencia, Spain
² Instituto de Bioquímica y Biología Molecular (IBBM), Universidad Nacional de La Plata (UNLP), La Plata, Argentina
³ CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), Argentina
⁴ Dipartimento di Arboricoltura, Botanica e Patología Vegetale, Università degli Studi di Napoli Federico II, Portici, Napoli, Italy
⁵ Comisión de Investigaciones Científicas de Buenos Aires, Buenos Aires, Argentina

Correspondence
José Guerri
jguerri{at}ivia.es

Citrus psorosis virus (CPsV), the type species of genus Ophiovirus, has a segmented, negative-stranded RNA genome. We examined the population structure and genetic variation of CPsV in three coding regions located in RNAs 1, 2 and 3, analysing 22 isolates from Argentina, California, Florida, Italy and Spain. Most isolates contained a predominant sequence and some minor variants. Estimations of the genetic diversity and phylogenetic clustering of isolates disclosed two populations, one comprising isolates from Spain, Italy, Florida and California and the other including the Argentinean isolates. Isolate CPV-4 (from Texas) included for comparison was distant from both groups, suggesting that it belongs to a third group. The low ratio between non-synonymous and synonymous nucleotide substitutions indicated strong selection for amino acid sequence conservation, particularly in the coat protein gene. Incongruent phylogenetic relationships in different genomic regions suggested that exchange of genomic segments may have contributed to CPsV evolution.

The GenBank/EMBL/DDBJ accession numbers of the CPsV sequences determined in this study are AM268273–AM268314 (region R1), AM235891–AM235946 (region R2) and AM235947–AM236001 (region R3).

Supplementary material is available in JGV Online.