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Molecular characterization of the largest mycoviral-like double-stranded RNAs associated with Amasya cherry disease, a disease of presumed fungal aetiology

S. Açkgöz⁶

¹ Division of Biology, Faculty of Natural Sciences, Sir Alexander Fleming Building, Imperial College London, Imperial College Road, London SW7 2AZ, UK
² Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, 46022 Valencia, Spain
³ Dipartimento di Arboricoltura, Botanica e Patologia Vegetale, Università di Napoli, 80055 Portici, Italy
⁴ Istituto di Virologia Vegetale del CNR, Sezione di Bari, 70126 Bari, Italy
⁵ Tekirdag Ziraat Fakültesi, Trakya Universitesi, 59030 Tekirdag, Turkey
⁶ Adnan Menderes University, Agricultural Faculty, Plant Pathology Department, 09100 Aydin, Turkey

Correspondence
R. H. A. Coutts
r.coutts{at}imperial.ac.uk

The sequence of the four large (L) double-stranded RNAs (dsRNAs) associated with Amasya cherry disease (ACD), which has a presumed fungal aetiology, is reported. ACD L dsRNAs 1 (5121 bp) and 2 (5047 bp) potentially encode proteins of 1628 and 1620 aa, respectively, that are 37 % identical and of unknown function. ACD L dsRNAs 3 (4458 bp) and 4 (4303 bp) potentially encode proteins that are 68 % identical and contain the eight motifs conserved in RNA-dependent RNA polymerases (RdRp) of dsRNA mycoviruses, having highest similarity with those of members of the family Totiviridae. Both terminal regions share extensive conservation in all four RNAs, suggesting a functional relationship between them. As ACD L dsRNAs 1 and 2 do not encode RdRps, both are probably replicated by those from either ACD L dsRNA 3 or 4. Partial characterization of the equivalent L dsRNAs 3 and 4 associated with cherry chlorotic rusty spot revealed essentially identical sequences.

The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are AM085136, AM085137, AM085134 and AM085135 for full-length ACD-associated large dsRNAs 1, 2, 3 and 4, respectively, and AM181139–AM181141 and AM181142 for partial-length CCRS-associated large dsRNAs 3 and 4, respectively.

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