HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Timmusk, S. Articles by Berg, M. Search for Related Content PubMed PubMed Citation Articles by Timmusk, S. Articles by Berg, M. Agricola Articles by Timmusk, S. Articles by Berg, M.

Porcine circovirus type 2 replicase binds the capsid protein and an intermediate filament-like protein

¹ Department of Molecular Biosciences, Section of Veterinary Immunology and Virology, Swedish University of Agricultural Sciences, Box 588, S-751 23 Uppsala, Sweden
² Department of Biomedical Sciences and Veterinary Public Health, Section of Parasitology and Virology, Swedish University of Agricultural Sciences, Box 588, S-751 23 Uppsala, Sweden

Correspondence
Sirje Timmusk
Sirje.Timmusk{at}vmm.slu.se

Porcine circovirus type 2 (PCV2) is an important porcine pathogen that establishes persistent subclinical infections but may, on activation, contribute to the development of post-weaning multisystemic wasting syndrome (PMWS). This disease is characterized by weight loss, respiratory or digestive disorders and enlarged lymph nodes with lymphocyte depletion. The molecular mechanisms behind the development of the disease are completely unknown. In order to clarify functions of the different viral proteins and, if possible, to connect these new findings to molecular mechanisms behind the pathogenesis or the viral life cycle, a bacterial two-hybrid screening of a porcine expression library from PK-15A cells was conducted. Using viral proteins corresponding to ORFs 1, 2, 3 and 4 as bait, a number of interactions were identified and two of them were chosen for further characterization. GST pull-down assays confirmed that viral replicase (Rep) interacted with an intermediate filament protein, similar to human syncoilin, and with the transcriptional regulator c-myc. Furthermore, interactions of the viral proteins to each other revealed an interaction between PCV2 Rep and the capsid (Cap) protein and Cap to itself.

This article has been cited by other articles:

				S. Timmusk, E. Merlot, T. Lovgren, L. Jarvekulg, M. Berg, and C. Fossum Regulator of G protein signalling 16 is a target for a porcine circovirus type 2 protein J. Gen. Virol., October 1, 2009; 90(10): 2425 - 2436. [Abstract] [Full Text] [PDF]