J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 87 (2006), 3425-3431; DOI 10.1099/vir.0.82252-0

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary figures
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, M. J.
Right arrow Articles by Paek, K.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, M. J.
Right arrow Articles by Paek, K.-H.
Agricola
Right arrow Articles by Kim, M. J.
Right arrow Articles by Paek, K.-H.
© 2006 Society for General Microbiology

Short Communication

Arabidopsis tonoplast proteins TIP1 and TIP2 interact with the cucumber mosaic virus 1a replication protein

Min Jung Kim{dagger}, Hwa Ran Kim{dagger} and Kyung-Hee Paek

School of Life Sciences and Biotechnology, Korea University, 1 5-ga Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of Korea

Correspondence
Kyung-Hee Paek
khpaek95{at}korea.ac.kr

The cucumber mosaic virus (CMV) replication complex has previously been shown to associate with cellular membranes. However, it remains unknown whether any host factors participate in this process. In this study, five groups of Arabidopsis tonoplast intrinsic protein (TIP) genes were isolated and the proteins they encoded were evaluated with regard to their interactions with CMV proteins. TIP1 and TIP2 were found to interact with the CMV 1a protein in the Sos recruitment system, whereas no interactions with the other three TIP subgroups were observed in this assay. The interaction of CMV 1a with the TIP1 and TIP2 proteins was confirmed via co-immunoprecipitation assays. Additionally, CMV 1a co-localized with TIP1 and TIP2 in transfected Arabidopsis protoplasts. The findings of this study suggest that members of two TIP subfamilies might affect CMV replication via interaction with CMV 1a in the tonoplasts.

Figures showing interaction analyses of Arabidopsis TIPs and CMV or TMV proteins using a yeast two-hybrid system, and the results of liquid assays for quantification of beta-galactosidase activity are available as supplementary material in JGV Online.

{dagger}These authors contributed equally to this work.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2006 by the Society for General Microbiology.