HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Table Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bucher, E. Articles by Prins, M. Search for Related Content PubMed PubMed Citation Articles by Bucher, E. Articles by Prins, M. Agricola Articles by Bucher, E. Articles by Prins, M.

Multiple virus resistance at a high frequency using a single transgene construct

Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands

Correspondence
Marcel Prins
marcel.prins{at}wur.nl

RNA silencing is a natural antiviral defence in plants, which can be exploited in transgenic plants for preprogramming virus recognition and ensuring enhanced resistance. By arranging viral transgenes as inverted repeats it is thus possible to obtain strong repression of incoming viruses. Due to the high sequence specificity of RNA silencing, this technology has hitherto been limited to the targeting of single viruses. Here it is shown that efficient simultaneous targeting of four different tospoviruses can be achieved by using a single small transgene based on the production of minimal sized chimaeric cassettes. Due to simultaneous RNA silencing, as demonstrated by specific siRNA accumulation, the transgenic expression of these cassettes rendered up to 82 % of the transformed plant lines heritably resistant against all four viruses. Thus RNA silencing can be further improved for high frequency multiple virus resistance by combining small RNA fragments from a series of target viruses.

A table of primer sequences used in fusion-PCR is available as supplementary material in JGV Online.

This article has been cited by other articles:

				M. Dafny-Yelin, S.-M. Chung, E. L. Frankman, and T. Tzfira pSAT RNA Interference Vectors: A Modular Series for Multiple Gene Down-Regulation in Plants Plant Physiology, December 1, 2007; 145(4): 1272 - 1281. [Abstract] [Full Text] [PDF]

				S. G. Ribeiro, H. Lohuis, R. Goldbach, and M. Prins Tomato Chlorotic Mottle Virus Is a Target of RNA Silencing but the Presence of Specific Short Interfering RNAs Does Not Guarantee Resistance in Transgenic Plants J. Virol., February 15, 2007; 81(4): 1563 - 1573. [Abstract] [Full Text] [PDF]