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J Gen Virol 87 (2006), 445-449; DOI 10.1099/vir.0.81565-0

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© 2006 Society for General Microbiology

Short Communication

Biological properties of Beet mild yellowing virus derived from a full-length cDNA clone

Dirk Stephan and Edgar Maiss

Institute of Plant Diseases and Plant Protection, University of Hannover, Herrenhäuser Straße 2, D 30419 Hannover, Germany

Correspondence
Dirk Stephan
stephan{at}ipp.uni-hannover.de

A German isolate of Beet mild yellowing virus (BMYV-IPP) was used for RT-PCR-based construction of the first infectious full-length cDNA clone of the virus (BMYVfl). The complete genomic sequence was determined and displayed high similarity to the French isolate BMYV-2ITB. The host range of BMYVfl was examined by agroinoculation and aphid transmission. Both methods lead to systemic infections in Beta vulgaris, Nicotiana benthamiana, N. clevelandii, N. hesperis, Capsella bursa-pastoris and Lamium purpureum. Immunological investigation by tissue-print immunoassay (TPIA) of agroinoculated plant tissues revealed only local infections restricted to the agroinoculated mesophyll tissues in some plant species. In Nicotiana glutinosa and N. edwardsonii, BMYV was not found in either the agroinoculated tissue or distant tissues by TPIA. So far, BMYVfl agroinoculation did not extend or confine the BMYV host range known from aphid transmission experiments but it did describe new local hosts for BMYV.

The GenBank/EMBL/DDBJ accession number for the sequence of the infectious full-length cDNA clone BMYVfl is DQ132996.






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