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J Gen Virol 87 (2006), 697-704; DOI 10.1099/vir.0.81545-0

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© 2006 Society for General Microbiology

Putative neutralization epitopes and broad cross-genotype neutralization of Hepatitis E virus confirmed by a quantitative cell-culture assay

Suzanne U. Emerson1, Pilar Clemente-Casares1, Nasser Moiduddin1, Vidya A. Arankalle2, Udana Torian1 and Robert H. Purcell1

1 Molecular Hepatitis and Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 50 South Drive MSC-8009, Bethesda, MD 20892-8009, USA
2 Hepatitis Division, National Institute of Virology, Pune, India

Correspondence
Suzanne U. Emerson
semerson{at}niaid.nih.gov

Monolayers of Hep G2/C3A cells were inoculated with genotype 1 Hepatitis E virus (HEV) mixed with either anti-HEV or an appropriate control. After 5 or 6 days, cell monolayers were stained with anti-HEV and infected cells were identified by immunofluorescence microscopy and counted. Anti-HEV from vaccinated or infected rhesus monkeys neutralized the virus, as did mAbs that recognized epitopes on the C terminus of a recombinant vaccine protein. Antibodies were broadly cross-reactive, since convalescent serum from animals infected with any one of the four mammalian genotypes all neutralized the genotype 1 virus.




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