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Tobacco mosaic virus (TMV) and potato virus X (PVX) coat proteins confer heterologous interference to PVX and TMV infection, respectively

¹ Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, USA
² Instituto de Biotecnología, INTA Castelar, Las Cabañas y Los Reseros, B1712WAA Buenos Aires, Argentina

Correspondence
R. N. Beachy
RnBeachy{at}danforthcenter.org

Replication of Potato virus X (PVX) was reduced in transgenic protoplasts that accumulated wild-type coat protein (CP^WT) of Tobacco mosaic virus (TMV) or a mutant CP, CP^T42W, that produced highly ordered states of aggregation, including pseudovirions. This reaction is referred to as heterologous CP-mediated resistance. However, protoplasts expressing a CP mutant that abolished aggregation and did not produce pseudovirions, CP^T28W, did not reduce PVX replication. Similarly, in transgenic tobacco plants producing TMV CP^WT or CP^T42W, there was a delay in local cell-to-cell spread of PVX infection that was not observed in CP^T28W plants or in non-transgenic plants. The results suggest that the quaternary structure of the TMV CP regulates the mechanism(s) of heterologous CP-mediated resistance. Similarly, transgenic protoplasts that produced PVX CP conferred transient protection against infection by TMV RNA. Transgenic plants that accumulated PVX CP reduced the cell-to-cell spread of infection and resulted in a delay in systemic infection following inoculation with TMV or TMV RNA. Heterologous CP-mediated resistance was characterized by a brief delay in systemic infection, whilst homologous CP-mediated resistance conferred reduced or no systemic infection.

This article has been cited by other articles:

				A. A. Bazzini, H. E. Hopp, R. N. Beachy, and S. Asurmendi Infection and coaccumulation of tobacco mosaic virus proteins alter microRNA levels, correlating with symptom and plant development PNAS, July 17, 2007; 104(29): 12157 - 12162. [Abstract] [Full Text] [PDF]