HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yu, G. Articles by Cheng, G. Search for Related Content PubMed PubMed Citation Articles by Yu, G. Articles by Cheng, G. Agricola Articles by Yu, G. Articles by Cheng, G.

Functional disruption of the prion protein gene in cloned goats

¹ Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Graduate School of Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai, 200031, China
² Shanghai Transgenic Research Center, 88 Cai-Lun Road, Shanghai 201203, China
³ Shanghai Genon Bio-Engineering Co. Ltd, 88 Cai-Lun Road, Shanghai 201203, China

Correspondence
Guoxiang Cheng
chenggx{at}cngenon.com

The cellular prion protein (PrP^C), a membrane glycoprotein anchored to the outer surface of neurons, lymphocytes and other cells, is associated directly with the pathogenesis of the transmissible spongiform encephalopathies (TSEs) occurring mainly in humans, cattle, sheep and goats. Although mice lacking PrP^C develop and reproduce normally and are resistant to scrapie infection, large animals lacking PrP^C, especially those species in which TSE occurs naturally, are currently not available. Here, five live PRNP^+/– goats cloned by gene targeting are reported. Detailed RNA-transcription and protein-expression analysis of one PRNP^+/– goat showed that one allele of the caprine PRNP gene had been disrupted functionally. No gross abnormal development or behaviour could be seen in these PRNP^+/– goats up to at least 3 months of age. These heterozygous PRNP^+/– goats are ready to be used in producing homozygous PRNP^–/– goats in which no PrP^C should be expressed.

This article has been cited by other articles:

				C. S. Rogers, W. M. Abraham, K. A. Brogden, J. F. Engelhardt, J. T. Fisher, P. B. McCray Jr., G. McLennan, D. K. Meyerholz, E. Namati, L. S. Ostedgaard, et al. The porcine lung as a potential model for cystic fibrosis Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L240 - L263. [Abstract] [Full Text] [PDF]