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Analysis of the entire nucleotide sequence of hepatitis B virus genotype B in the Philippines reveals a new subgenotype of genotype B

¹ Division of Gastroenterology, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan
² Department of Gastroenterology, St Luke's Medical Center, Quezon, The Philippines
³ Tohoku University School of Health Sciences and Comprehensive Research and Education Center for Planning of Drug Development and Clinical Evaluation, Sendai, Japan
⁴ Department of Internal Medicine, Mizusawa General Hospital, Iwate, Japan

Correspondence
Hirofumi Niitsuma
hbvdna{at}ma.mni.ne.jp

The entire nucleotide sequences were determined for hepatitis B virus (HBV) genotype B (HBV/B) genomes extracted from five patients in the Philippines and designated GenBank AB219426 [GenBank] , AB219427 [GenBank] , AB219428 [GenBank] , AB219429 [GenBank] and AB219430 [GenBank] . The serotype of the first four isolates was ayw and that of GenBank AB219430 [GenBank] was adw. Divergences of entire sequences were 1·0–2·0 % between the first four isolates and 3·8–4·2 % between these four and GenBank AB219430 [GenBank] . Phylogenetic-tree analysis revealed that, worldwide, HBV/B comprises five subgenotypes: B1, B2, B3, B4 and the new Philippines group, designated B5. Divergences of the entire genome sequences between four isolates in subgenotype B5 and isolates from other countries (subgenotypes) were 4·4–4·8 % with Vietnam (B4), 2·9–3·5 % with Indonesia (B3), 4·7–5·1 % with China (B2) and 5·4–6·0 % with Japan (B1). Similarly, GenBank AB219430 [GenBank] showed the lowest divergences: 3·4 % with the isolate from Indonesia (B3), 5·0 % with Vietnam (B4), 5·4 % with China (B2) and 6·1 % with Japan (B1). This is the first report of entire nucleotide sequences of HBV/B from the Philippines and the results show that these sequences belong to a new subgenotype, B5. The present study identified that HBV/B isolates throughout the world are divided genetically into five subgenotypes, the relationships between geographical distances and the genetic distances of HBV/B being well-correlated.

The GenBank/EMBL/DDBJ accession numbers for the complete-genome sequences described in this paper are AB219426 [GenBank] –AB219430 [GenBank] .

A table showing a comparison of nucleotide similarities in the precore and core gene of the present five isolates from the Philippines and representative HBV/B isolates from four other subgenotypes, B1–B4, with isolates from HBV/C and B1 is available in JGV Online.

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