HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhang, Q. Articles by Zhou, R. Search for Related Content PubMed PubMed Citation Articles by Zhang, Q. Articles by Zhou, R. Agricola Articles by Zhang, Q. Articles by Zhou, R.

Comparative genomic analysis of two strains of human adenovirus type 3 isolated from children with acute respiratory infection in southern China

¹ State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
² Central Laboratory, Guangzhou Children's Hospital, Guangzhou 510120, China
³ South China Sea Institute of Oceanology, LED, Chinese Academy of Sciences, Guangzhou 510301, China
⁴ Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China

Correspondence
Chuyu Zhang
Zhang_whu{at}yahoo.com.cn
Rong Zhou
zhou3218{at}yahoo.com

Human adenovirus type 3 (HAdV-3) is a causative agent of acute respiratory disease, which is prevalent throughout the world, especially in Asia. Here, the complete genome sequences of two field strains of HAdV-3 (strains GZ1 and GZ2) isolated from children with acute respiratory infection in southern China are reported (GenBank accession nos DQ099432 [GenBank] and DQ105654 [GenBank] , respectively). The genomes were 35 273 bp (GZ1) and 35 269 bp (GZ2) and both had a G+C content of 51 mol%. They shared 99 % nucleotide identity and the four early and five late regions that are characteristic of human adenoviruses. Thirty-nine protein- and two RNA-coding sequences were identified in the genome sequences of both strains. Protein pX had a predicted molecular mass of 8.3 kDa in strain GZ1; this was lower (7.6 kDa) in strain GZ2. Both strains contained 10 short inverted repeats, in addition to their inverted terminal repeats (111 bp). Comparative whole-genome analysis revealed 93 mismatches and four insertions/deletions between the two strains. Strain GZ1 infection produced a typical cytopathic effect, whereas strain GZ2 did not; non-synonymous substitutions in proteins of GZ2 may be responsible for this difference.

The GenBank/EMBL/DDBJ accession numbers for the human adenovirus type 3 strain GZ1 and GZ2 sequences reported in this paper are DQ099432 [GenBank] and DQ105654 [GenBank] , respectively.

This article has been cited by other articles:

				Z. Zhu, Y. Zhang, S. Xu, P. Yu, X. Tian, L. Wang, Z. Liu, L. Tang, N. Mao, Y. Ji, et al. Outbreak of Acute Respiratory Disease in China Caused by B2 Species of Adenovirus Type 11 J. Clin. Microbiol., March 1, 2009; 47(3): 697 - 703. [Abstract] [Full Text] [PDF]