HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary material Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mukha, D. V. Articles by Schal, C. Search for Related Content PubMed PubMed Citation Articles by Mukha, D. V. Articles by Schal, C. Agricola Articles by Mukha, D. V. Articles by Schal, C.

Characterization of a new densovirus infecting the German cockroach, Blattella germanica

¹ Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow 119991, Russia
² Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
³ Department of Entomology, 219 Hodson Hall, 1980 Folwell Avenue, University of Minnesota, St Paul, MN 55108, USA
⁴ Department of Entomology and W. M. Keck Center for Behavioural Biology, North Carolina State University, Raleigh, NC 27695, USA

Correspondence
D. V. Mukha
myxa{at}vigg.ru

A new DNA virus (Parvoviridae: Densovirinae, Densovirus) was isolated and purified from descendants of field-collected German cockroaches, Blattella germanica. Viral DNA and cockroach tissues infected with B. germanica densovirus (BgDNV) were examined by electron microscopy. Virus particles, about 20 nm in diameter, were observed both in the nucleus and in the cytoplasm of infected cells. Virus DNA proved to be a linear molecule of about 1.2 µm in length. BgDNV isolated from infected cockroaches infected successfully and could be maintained in BGE-2, a B. germanica cell line. The complete BgDNV genome was sequenced and analysed. Five open reading frames (ORFs) were detected in the 5335 nt sequence: two ORFS that were on one DNA strand encoded structural capsid proteins (69.7 and 24.8 kDa) and three ORFs that were on the other strand encoded non-structural proteins (60.2, 30.3 and 25.9 kDa). Three putative promoters and polyadenylation signals were identified. Structural analysis of the inverted terminal repeats revealed the presence of extended palindromes. The genome structure of BgDNV was compared with that of other members of the family Parvoviridae; the predicted amino acid sequences were aligned and subjected to phylogenetic analyses.

The GenBank/EMBL/DDBJ accession number for the BgDNV genome sequence described in this paper is AY189948 [GenBank] .

Supplementary material is available in JGV Online.

This article has been cited by other articles:

				E. Baquerizo-Audiot, A. Abd-Alla, F.-X. Jousset, F. Cousserans, P. Tijssen, and M. Bergoin Structure and Expression Strategy of the Genome of Culex pipiens Densovirus, a Mosquito Densovirus with an Ambisense Organization J. Virol., July 1, 2009; 83(13): 6863 - 6873. [Abstract] [Full Text] [PDF]