HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary material Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Timchenko, T. Articles by Gronenborn, B. Search for Related Content PubMed PubMed Citation Articles by Timchenko, T. Articles by Gronenborn, B. Agricola Articles by Timchenko, T. Articles by Gronenborn, B.

Infectivity of nanovirus DNAs: induction of disease by cloned genome components of Faba bean necrotic yellows virus

M. Aronson^1,

J. C. Vega-Arreguín^1,

B. C. Ramirez^1,

¹ Institut des Sciences du Végétal, CNRS, Avenue de la Terrasse, Bât. 23, 91198 Gif sur Yvette, France
² Biologische Bundesanstalt für Land und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und Biologische Sicherheit, Messeweg 11-12, 38104 Braunschweig, Germany

Correspondence
T. Timchenko
taniat{at}isv.cnrs-gif.fr

Circumstantial evidence suggests that the genome of Faba bean necrotic yellows virus (FBNYV), a nanovirus, consists of eight distinct, circular, single-stranded DNAs, each of about 1 kb and encoding only one protein. Here, the use of cloned full-length FBNYV DNAs for reproducing FBNYV-like symptoms in Vicia faba, the principal natural host of FBNYV, is reported. Characteristic symptoms of FBNYV infection were obtained in faba bean plants following biolistic DNA delivery or agroinoculation with all eight FBNYV DNAs. Although the eight different DNAs have been invariably detected in field samples infected with the various geographical FBNYV isolates, experimental infection with different combinations of fewer than eight DNAs also led to typical FBNYV symptoms. Even only five genome components, DNA-R, DNA-S, DNA-M, DNA-U1 and DNA-U2, were sufficient for inducing disease symptoms in V. faba upon agroinoculation. Symptomatic plants agroinoculated or bombarded with eight DNAs contained typical FBNYV virions; however, the virus was not transmitted by Aphis craccivora or Acyrthosiphon pisum, two efficient aphid vectors of FBNYV.

Supplementary material is available in JGV Online.

Present address: Ambassade de France, 00180 Helsinki, Finland.

Present address: CINVESTAV, Unidad Irapuato, 36500 Irapuato, Guanajuato, Mexico.

Present address: Institut Pasteur, 75724 Paris Cedex 15, France.

This article has been cited by other articles:

				I. Grigoras, T. Timchenko, and B. Gronenborn Transcripts encoding the nanovirus master replication initiator proteins are terminally redundant J. Gen. Virol., February 1, 2008; 89(2): 583 - 593. [Abstract] [Full Text] [PDF]

				K. Dreher and J. Callis Ubiquitin, Hormones and Biotic Stress in Plants Ann. Bot., May 1, 2007; 99(5): 787 - 822. [Abstract] [Full Text] [PDF]

				S. Lageix, O. Catrice, J.-M. Deragon, B. Gronenborn, T. Pelissier, and B. C. Ramirez The Nanovirus-Encoded Clink Protein Affects Plant Cell Cycle Regulation through Interaction with the Retinoblastoma-Related Protein J. Virol., April 15, 2007; 81(8): 4177 - 4185. [Abstract] [Full Text] [PDF]

				J.-M. Hu, H.-C. Fu, C.-H. Lin, H.-J. Su, and H.-H. Yeh Reassortment and Concerted Evolution in Banana Bunchy Top Virus Genomes J. Virol., February 15, 2007; 81(4): 1746 - 1761. [Abstract] [Full Text] [PDF]