HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zahn, A. Articles by Allain, J.-P. Search for Related Content PubMed PubMed Citation Articles by Zahn, A. Articles by Allain, J.-P. Agricola Articles by Zahn, A. Articles by Allain, J.-P.

Hepatitis C virus interacts with human platelet glycoprotein VI

¹ Division of Transfusion Medicine, Department of Haematology, University of Cambridge, Cambridge CB2 2PT, UK
² National Blood Service, Long Road, Cambridge CB2 2PT, UK

Correspondence
Jean-Pierre Allain
jpa1000{at}cam.ac.uk

Hepatitis C virus (HCV) interacts with human platelets in vivo as a potential transport of infectious virions to the target liver. The binding of native viral particles with the platelet membrane glycoprotein VI (GPVI) was analysed. A consistent interaction between HCV from plasma or after purification by two different methods and the recombinant extracellular immunoglobulin (Ig)-like domains of human GPVI (hD1D2) was observed with two independent experimental approaches: pull-down and ELISA assays. Between 2 and 7 % of HCV particles were specifically bound to hD1D2. The binding was inhibited by an anti-hD1D2 in a dose-dependent manner. Human D1D2 interaction with HCV was significantly higher than the murine D1D2, supporting the specificity of the interaction and to the single human domains (D1 and D2), suggesting that both Ig-like domains of the molecule are required for efficient binding. GPVI may be a platelet surface ligand for HCV playing a role in viral transport and persistence.

This article has been cited by other articles:

				T. Bruns, K. Steinmetzer, E. Ermantraut, and A. Stallmach Hepatitis C Virus RNA Quantitation in Venous and Capillary Small-Volume Whole-Blood Samples J. Clin. Microbiol., October 1, 2009; 47(10): 3231 - 3240. [Abstract] [Full Text] [PDF]

				K. Ghosh, S. Gangodkar, P. Jain, S. Shetty, S. Ramjee, P. Poddar, and A. Basu Imaging the interaction between dengue 2 virus and human blood platelets using atomic force and electron microscopy J. Electron Microsc. (Tokyo), June 1, 2008; 57(3): 113 - 118. [Abstract] [Full Text] [PDF]