HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary figure Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kondo, H. Articles by Tamada, T. Search for Related Content PubMed PubMed Citation Articles by Kondo, H. Articles by Tamada, T. Agricola Articles by Kondo, H. Articles by Tamada, T.

Orchid fleck virus is a rhabdovirus with an unusual bipartite genome

Takanori Maeda^1,

¹ Research Institute for Bioresources, Okayama University, Kurashiki 710-0046, Japan
² Asian Center for Bioresources and Environmental Sciences, University of Tokyo, Tokyo 113-0032 Japan

Correspondence
Hideki Kondo
hkondo{at}rib.okayama-u.ac.jp

Orchid fleck virus (OFV) has an unusual bipartite negative-sense RNA genome with clear sequence similarities to those of nucleorhabdoviruses. The OFV genome consists of two single-stranded RNA molecules, RNA1 and RNA2 that are 6413 and 6001 nt long, respectively, with open reading frame (ORF) information in the complementary sense. RNA1 encodes 49 (ORF1), 26 (ORF2), 38 (ORF3), 20 (ORF4) and 61 kDa (ORF5) proteins, and RNA2 encodes a single protein of 212 kDa (ORF6). ORF1, ORF5 and ORF6 proteins had significant similarities (21–38 % identity) to the nucleocapsid protein (N), glycoprotein (G) and polymerase (L) gene products, respectively, of other rhabdoviruses, especially nucleorhabdoviruses, whereas ORF2, ORF3 and ORF4 proteins had no significant similarities to other proteins in the international databases. Similarities between OFV and rhabdoviruses were also found in the sequence complementarity at both termini of each RNA segment (the common terminal sequences are 3'-UGUGUC---GACACA-5'), the conserved intergenic sequences and in being negative sense. It was proposed that a new genus Dichorhabdovirus in the family Rhabdoviridae of the order Mononegavirales should be established with OFV as its prototype member and type species.

The GenBank/EMBL/DDBJ accession numbers of the sequences reported in this paper are AB244417 and AB244418.

Hydrophobicity plots of the G proteins of OFV and RYSV are available as supplementary material in JGV Online.

Present address: College of Bioresource Sciences, Nihon University, Fujisawa 252-8510, Japan.