J Gen Virol Try IJSEM Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 87 (2006), 2433-2441; DOI 10.1099/vir.0.81491-0

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Everest, S. J.
Right arrow Articles by Jackman, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Everest, S. J.
Right arrow Articles by Jackman, R.
Agricola
Right arrow Articles by Everest, S. J.
Right arrow Articles by Jackman, R.
© 2006 Society for General Microbiology

No abnormal prion protein detected in the milk of cattle infected with the bovine spongiform encephalopathy agent

Sally J. Everest1, Leigh T. Thorne1, Jeremy A. Hawthorn1, Russell Jenkins1, Clare Hammersley1, Andrew M. Ramsay1, Stephen A. Hawkins1, Lindsay Venables1, Linda Flynn1, Robin Sayers1, John Kilpatrick2, Amanda Sach3, James Hope4 and Roy Jackman1

1 Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK
2 ADAS Rosemaund, Preston Wynne, Hereford HR1 3PG, UK
3 ADAS Defra Drayton, Stratford-upon-Avon, Warwickshire CV37 9RQ, UK
4 VLA Lasswade, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK

Correspondence
James Hope
j.hope{at}vla.defra.gsi.gov.uk

Milk specimens were collected from lactating cows that had previously been challenged with bovine spongiform encephalopathy (BSE)-infected brain at 4–6 months of age. One group of 10 animals received a single oral dose of 100 g, a second group received 1 g and the third was made up of unexposed controls. The cows were inseminated artificially, and calved at approximately 2 years of age and annually thereafter. Milking was done within the first week following calving and at 10-weekly intervals during the lactation period. Specimens were centrifuged to obtain a fraction enriched for somatic cells and these fractions were analysed for disease-associated, abnormal prion protein (PrPBSE) by using a modified commercial BSE ELISA and a different confirmatory assay. No abnormal prion protein has so far been identified in the cell fraction of milk from cattle incubating BSE by using these methods at their limits of detection.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2006 by the Society for General Microbiology.