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Complete sequence and analysis of the ovine herpesvirus 2 genome

¹ Department of Veterinary Pathology, University of Edinburgh, Edinburgh, UK
² Institute of Virology, University of Zurich, Zurich, Switzerland
³ Division of Medical Microbiology, School of Infection and Host Defence, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA, UK
⁴ Division of Virology, Moredun Research Institute, Edinburgh, UK

Correspondence
James P. Stewart
j.p.stewart{at}liv.ac.uk

Ovine herpesvirus 2 (OvHV-2) is endemic in sheep populations worldwide and causes malignant catarrhal fever (MCF), a lymphoproliferative disease, in cattle, bison and deer. OvHV-2 has been placed in the gammaherpesvirus subfamily and is related closely to Alcelaphine herpesvirus 1 (AlHV-1). Here, the cloning, sequencing and analysis of the complete OvHV-2 genome derived from a lymphoblastoid cell line from an affected cow (BJ1035) are reported. The unique portion of the genome consists of 130 930 bp, with a mean G+C content of 52 mol%. The unique DNA is flanked by multiple copies of terminal repeat elements 4205 bp in length, with a mean G+C content of 72 mol%. Analysis revealed 73 open reading frames (ORFs), the majority (62) of which showed homology to other gammaherpesvirus genes. A further subset of nine ORFs is shared with only the related AlHV-1. Three ORFs are entirely unique to OvHV-2, including a spliced homologue of cellular interleukin-10 that retains the exon structure of the cellular gene. The sequence of OvHV-2 is a critical first step in the study of the pathogenesis and treatment of MCF.

The GenBank/EMBL/DDBJ accession number for the sequence reported in this paper is AY839756.

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