HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yaegashi, H. Articles by Yoshikawa, N. Search for Related Content PubMed PubMed Citation Articles by Yaegashi, H. Articles by Yoshikawa, N. Agricola Articles by Yaegashi, H. Articles by Yoshikawa, N.

Apple chlorotic leaf spot virus 50 kDa movement protein acts as a suppressor of systemic silencing without interfering with local silencing in Nicotiana benthamiana

¹ The United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550, Japan
² The 21st Century Center of Excellence Program, Iwate University, Morioka 020-8550, Japan
³ Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan
⁴ Kyoto Prefectural Institute of Agricultural Biotechnology, Soraku-gun, Kyoto 619-0244, Japan
⁵ Graduate School of Life and Environmental Sciences, University of Osaka Prefecture, Sakai 599-8531, Japan

Correspondence
Nobu Yoshikawa
Yoshikawa{at}iwate-u.ac.jp

Apple chlorotic leaf spot virus (ACLSV) is the type species of the genus Trichovirus and its single-stranded, plus-sense RNA genome encodes a 216 kDa protein (P216) involved in replication, a 50 kDa movement protein (P50) and a 21 kDa coat protein (CP). In this study, it was investigated whether these proteins might have RNA silencing-suppressor activities by Agrobacterium-mediated transient assay in the green fluorescent protein-expressing Nicotiana benthamiana line 16c. The results indicated that none of these proteins could suppress local silencing in infiltrated leaves. However, systemic silencing in upper leaves induced by both single- and double-stranded RNA could be suppressed by P50, but not by a frame-shift mutant of P50, P216 or CP. Moreover, when P50 was expressed separately from where silencing signals were generated in a leaf, systemic silencing in upper leaves was inhibited. Collectively, our data indicate that P50 acts as a suppressor of systemic silencing without interfering with local silencing, probably by inhibiting the movement of silencing signals.

This article has been cited by other articles:

				J. Jovel, M. Walker, and H. Sanfacon Recovery of Nicotiana benthamiana Plants from a Necrotic Response Induced by a Nepovirus Is Associated with RNA Silencing but Not with Reduced Virus Titer J. Virol., November 15, 2007; 81(22): 12285 - 12297. [Abstract] [Full Text] [PDF]

				H. Yaegashi, M. Isogai, H. Tajima, T. Sano, and N. Yoshikawa Combinations of two amino acids (Ala40 and Phe75 or Ser40 and Tyr75) in the coat protein of apple chlorotic leaf spot virus are crucial for infectivity J. Gen. Virol., September 1, 2007; 88(9): 2611 - 2618. [Abstract] [Full Text] [PDF]