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J Gen Virol 88 (2007), 71-76; DOI 10.1099/vir.0.82445-0

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© 2007 Society for General Microbiology

Short Communication

Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation

Victoria Sherwood1, Hans-Gerhard Burgert2, Yun-Hsiang Chen3, Sandeep Sanghera1, Socrates Katafigiotis1, Richard E. Randall3, Ian Connerton1 and Kenneth H. Mellits1

1 Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonnington Campus, Loughborough LE12 5RD, UK
2 Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
3 School of Biology, University of St Andrews, Fife KY16 9TS, UK

Correspondence
Kenneth H. Mellits
ken.mellits{at}nottingham.ac.uk

Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.

A supplementary figure showing increased adenovirus DNA replication in HAdV-40-infected MRC5-SV5/V cells is available in JGV Online.






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