HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nerome, R. Articles by Kurane, I. Search for Related Content PubMed PubMed Citation Articles by Nerome, R. Articles by Kurane, I. Agricola Articles by Nerome, R. Articles by Kurane, I.

Molecular epidemiological analyses of Japanese encephalitis virus isolates from swine in Japan from 2002 to 2004

¹ Department of Virology 1, National Institute of Infectious Diseases (NIID), Toyama, Shinjuku, Tokyo 162-8640, Japan
² Mitsubishi Kagaku Bio-Clinical Laboratories Inc., Tokyo, Japan
³ Mie Prefectural Institute of Public Health and Environmental Sciences, Mie, Japan
⁴ Kagawa Prefectural Institute of Environment and Health, Kagawa, Japan
⁵ Hiroshima Prefectural Institute of Health and Environment, Hiroshima, Japan
⁶ Chiba Prefectural Institute of Public Health, Chiba, Japan
⁷ Shizuoka Prefectural Institute of Environment and Hygiene, Shizuoka, Japan
⁸ Shizuoka Prefectural Seibu Meat Inspection Center, Shizuoka, Japan
⁹ Okinawa Prefectural Institute of Health and Environment, Okinawa, Japan
¹⁰ Ishikawa Prefectural Institute of Public Health and Environmental Science, Ishikawa, Japan
¹¹ The Public Health Institute of Kochi Prefecture, Kochi, Japan
¹² Tokyo Metropolitan Institute of Public Health, Tokyo, Japan
¹³ Kumamoto Prefectural Institute of Public Health and Environmental Science, Kumamoto, Japan

Correspondence
Ichiro Kurane
kurane{at}nih.go.jp

To characterize Japanese encephalitis virus (JEV) strains recently prevalent in Japan, JEV surveillance was performed in pigs from 2002 to 2004. Eleven new JEV isolates were obtained and compared with previous isolates from Japan and other Asian countries. All of the isolates were classified into genotype 1 by nucleotide sequence analysis of the E gene. Two new isolates with different levels of neurovirulence and neuroinvasiveness, but with only one nucleotide difference in the E gene, Sw/Mie/34/2004 and Sw/Mie/40/2004, were isolated at the same farm on the same day. Sw/Mie/40/2004 displayed higher neurovirulence and neuroinvasiveness in mice than the other four new isolates. Another new isolate, Sw/Hiroshima/25/2002, was neutralized by antiserum to Beijing-1 at a level similar to the homologous Beijing-1 strain, whilst seven other new isolates were neutralized at 10-fold-lower titres. However, there were no amino acid differences in the E protein among these eight isolates. The present study indicated that the 11 new JEV isolates were genetically similar, but biologically and serologically heterogeneous.

The GenBank/EMBL/DDBJ accession numbers for the E gene and 3' NTR sequences of the 14 JEV isolates determined in this study (– indicates ‘data not available’) are: Sw/Hiroshima/46/1998 (AB174837, –), Sw/Hiroshima/38/2000 (AB174838, –), Sw/Kagawa/24/2002 (AB112706, –), Sw/Kagawa/27/2002 (AB112707, AB231626 ), Sw/Hiroshima/25/2004 (AB231465, AB231621), Sw/Shizuoka/33/2002 (AB112703, AB231620), Sw/Shizuoka/39/2002 (AB112704, –), Sw/Chiba/88/2002 (AB112705, AB231622), Sw/Mie/41/2002 (AB112709, AB231623), Sw/Okinawa/285/2003 (AB238693, AB238694) Sw/Kagawa/35/2004 (AB231464, AB231627), Sw/Mie/34/2004 (AB231462, AB231624), Sw/Mie/40/2004 (AB231463, AB231625), JaTAn 1/94 (AB237171, AB237172).