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J Gen Virol 88 (2007), 2774-2779; DOI 10.1099/vir.0.83060-0

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Short Communication

Increased human immunodeficiency virus type 1 Env expression and antibody induction using an enhanced alphavirus vector

Mattias N. E. Forsell, Gerald M. McInerney, Pia Dosenovic, Åsa S. Hidmark, Christopher Eriksson, Peter Liljeström, Christoph Grundner and Gunilla B. Karlsson Hedestam

Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden, and Swedish Institute for Infectious Disease Control, SE-171 82 Solna, Sweden

Correspondence
Gunilla B. Karlsson Hedestam
Gunilla.Karlsson.Hedestam{at}ki.se

Viral vectors encoding heterologous vaccine antigens are potent inducers of cellular immune responses, but they are generally less efficient at stimulating humoral immunity. To improve the induction of antibody responses by Semliki Forest virus-based vaccines, a vector encoding a translation-enhancer element and a novel internal signal sequence for increased expression and secretion of soluble antigens was designed. Approximately tenfold more human immunodeficiency virus type 1 gp120 was secreted into culture supernatants of infected cells using the enhanced vector compared with the parental vector. This translated into a significant increase in gp120-specific antibodies in immunized mice, suggesting that antigen-expression levels from the parental vector are limiting for induction of antibody responses. These data encourage the use of the enhanced vector for elicitation of immune responses against heterologous antigens during vaccination.

{dagger}Present address: Department of Molecular and Cell Biology, University of California, Berkeley, CA 94270, USA.






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