HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary table Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wallis, C. M. Articles by Schneider, W. L. Search for Related Content PubMed PubMed Citation Articles by Wallis, C. M. Articles by Schneider, W. L. Agricola Articles by Wallis, C. M. Articles by Schneider, W. L.

Adaptation of plum pox virus to a herbaceous host (Pisum sativum) following serial passages

¹ Department of Plant Pathology, The Ohio State University, Columbus, OH 43210, USA
² USDA-ARS Foreign Disease–Weed Science Research Unit, 1301 Ditto Ave, Fort Detrick, MD 21702, USA
³ Department of Plant Pathology, Penn State University, Buckhout Laboratory, University Park, PA 16802, USA

Correspondence
William L. Schneider
William.Schneider{at}ars.usda.gov

Plum pox virus (PPV) populations from peaches are able to adapt consistently to herbaceous hosts, characterized by a reduction in time to symptom development, increases in inoculation efficiency and increased titres. PPV adaptation was studied by using pea (Pisum sativum) as an alternative host. Two isolates of PPV from peaches were inoculated and passaged in peas ten times using either aphid or mechanical inoculation, generating four independent passage lines. Mechanical-transmission efficiency from peach to pea improved from 3 % at passage 1 to 100 % by serial passage 4 on peas. Inoculation using aphid vectors required six to ten serial passages in pea to reach a peak of 50–60 % transmission efficiency. Sequence analyses of all four PPV population lines inoculated sequentially to pea identified a specific mutation occurring consistently in the NIb gene when compared with the same PPV isolates passaged in parallel in peach. The mutation allowed PPV to replicate up to 20 times faster in the new host. Pea-adapted strains of PPV at every passage were also tested for their ability to infect the original host, peach. Regardless of the number of previous passages, all pea-adapted PPV strains consistently infected peach at low levels using aphid inoculation.

The GenBank/EMBL/DDBJ accession numbers for the complete genome sequences of PPV isolates PENN3 and PENN4 are DQ465242 and DQ465243, respectively.

A supplementary table showing the primer pairs used in this study is available with the online version of this paper.