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A chimeric plum pox virus shows reduced spread and cannot compete with its parental wild-type viruses in a mixed infection

Christof Dietrich^1,

Qusai Al Abdallah^2,,

¹ German Collection of Microorganisms and Cell Cultures, Plant Virus Division, Inhoffenstr. 7b, 38124 Braunschweig, Germany
² Institute of Plant Diseases and Plant Protection, University of Hannover, Herrenhäuser Str. 2, 30419 Hannover, Germany

Correspondence
Christof Dietrich
cdi04{at}dsmz.de

The effect of a recombination event in the genomic 3' end on the biological properties and competitiveness of plum pox virus (PPV) was investigated. Therefore, a fragment spanning the coat protein (CP) coding region and a part of the 3' non-translated region of a non-aphid-transmissible strain of PPV (PPV-NAT) was replaced by the corresponding region of a PPV sour cherry isolate (PPV-SoC). The resulting chimera (PPV-NAT/SoC) caused severe symptoms in Nicotiana benthamiana, resembling those of PPV-NAT. In mixed infections with either of the parental viruses, the chimera PPV-NAT/SoC was less competitive. Labelling experiments with DsRed showed that PPV-NAT/SoC (PPV-NAT/SoC-red) moved more slowly from cell to cell than PPV-NAT (PPV-NAT-red). In mixed infections of PPV-NAT/SoC-red with a green fluorescent protein-expressing PPV-NAT (PPV-NAT-AgfpS), spatial separation of the viruses was observed. These data suggest that, in PPV infections, symptom severity and competitiveness are independent aspects and that spatial separation may contribute to the displacement of a recombinant virus.

These authors contributed equally to this work.

Present address: Leibniz Institute for Natural Product Research and Infection Biology, Department of Molecular and Applied Microbiology, Beutenbergstr. 11a, 07745 Jena, Germany.

A figure showing competition of PPV-NAT, PPV-NAT/SoC and PPV-SoC in different mixed infections during mechanical passaging is available with the online version of this paper.